品牌与标准化Issue(6)：22-24,3.DOI:10.3969/j.issn.1674-4977.2024.06.007
超高效液相色谱-串联质谱法测定鲜蛋中喹诺酮类化合物
Determination of Fluoroquinolones Residues in Fresh Eggs by UPLC-MS/MS
摘要
Abstract
A method for the simultaneous determination of Fluoroquinolones in fresh eggs by UPLC-MS/MS was established using QuEChERS pretreatment,and the sample pretreatment and chromatographic conditions are optimized.The sample is extracted using acidified acetonitrile,salting out,centrifuged to obtain the supernatant,dried under nitrogen in a 40℃ water bath,and after reconstitution,lipid soluble impurities were removed using n-hexane.The lower layer solution is filtered and analyzed using UPLC-MS/MS.The results shows that the correlation coefficient of fluoroquinolones between 2～100µg/L is greater than 0.99,with detection and quantification limits of 0.5 and 2µg/kg,respectively.The recovery rate is between 87.3％～109.1％,with a relative standard deviation of 1.3％～10.2％(n=6).Seven samples are detected for enrofloxacin using the established method.This method is simple,economical,and practical,suitable for the determination of quinolone compounds in fresh eggs.关键词
超高效液相色谱-串联质谱法/鲜蛋/喹诺酮/检测Key words
UPLC-MS/MS/fresh eggs/quinolones/detection引用本文复制引用
陈玉威,孙晓娟,周子人,高广慧..超高效液相色谱-串联质谱法测定鲜蛋中喹诺酮类化合物[J].品牌与标准化,2024,(6):22-24,3.基金项目
辽宁省应用基础研究计划项目(2022JH2/101300076):食品安全非靶向筛查及溯源识别关键技术研究. （2022JH2/101300076）
