肿瘤预防与治疗2024，Vol.37Issue(10)：823-830,8.DOI:10.3969/j.issn.1674-0904.2024.10.001
低强度聚焦超声联合顺铂对宫颈癌裸鼠皮下移植瘤抑制及增敏作用的实验研究
Experimental Study on the Inhibitory and Sensitizing Effects of Low-Inten-sity Focused Ultrasound Combined with Cisplatin on Subcutaneously Transplanted Cervical Cancer in Nude Mice
摘要
Abstract
Objective:To investigate the inhibitory and sensitizing effects of low-intensity focused ultrasound(LIFU)alone and LIFU combined with cisplatin on subcutaneously transplanted cervical cancer in nude mice.Methods:Human cervical cancer HeLa cells were inoculated into female BALB/c nude mice to establish a subcutaneous transplanted tumor model of cervical cancer.The successfully modeled mice were randomly assigned to the control group,the cisplatin group,the LIFU group,and the LIFU combined with cisplatin group(the combination group).After being treated,the general condition and tumor growth of the nude mice were observed.The pathological tissue and morphological changes in tumor cells were observed by HE staining,and the apoptosis of cervical cancer cells in different groups was detected by terminal deoxynucleotidyl transferase dUTP nick-end labeling(TUNEL)as-say.Results:The general condition of the nude mice in the LIFU group was good,while that in the cisplatin group was the worst.The HE staining results revealed that necrotic areas were found in the LIFU group,the cisplatin group and the combi-nation group,with a significant increase in the necrotic area in the cisplatin group compared to the LIFU group,and that the largest necrotic area was observed in the combination group.The tumor growth inhibition rates in the LIFU group,the cispla-tin group,and the combination group were 6.13％(P＜0.05),73.67％(P＜0.01)and 79.18％(P＜0.01)which were significantly different from that in the control group.The TUNEL assay revealed that the apoptosis indexes in the LIFU group,the cisplatin group and the combination group increased(P＜0.05,P＜0.01,P＜0.01),and the combination group showed the most obvious cell apoptosis.Conclusion:LIFU is a relatively safe treatment that can inhibit the growth of subcutaneously transplanted cervical cancer.LIFU combined with cisplatin can effectively improve its sensitivity to cisplatin,and enhance its inhibitory effect on cervical cancer cells.The combination therapy has a significantly better inhibitory effect on the growth of subcutaneously transplanted cervical cancer in nude mice than the single use of cisplatin.关键词
宫颈癌/低强度聚焦超声/顺铂Key words
Cervical cancer/Low-intensity focused ultrasound/Cisplatin分类
医药卫生引用本文复制引用
叶合欢,于梦婷,苏岚岚,杨会,秦娟..低强度聚焦超声联合顺铂对宫颈癌裸鼠皮下移植瘤抑制及增敏作用的实验研究[J].肿瘤预防与治疗,2024,37(10):823-830,8.基金项目
国家自然科学基金(编号:81860328) （编号:81860328）
贵州省卫生健康委员会科学技术基金(编号:gzwkj2024-183) This study was supported by National Natural Science Foundation of China(No.81860328),and by grants from Health Commission of Guizhou Province and Guizhou Science and Technology Department(No.gzwkj2024-183). （编号:gzwkj2024-183）
