基于过表达羧酸酯酶的人骨髓间充质干细胞联合LY2334737对膀胱癌的抑制作用研究OACSTPCD
Inhibitory Effect of Human Bone Mesenchymal Stromal Cells Overexpress-ing Carboxylesterases Combined with LY2334737 on Bladder Cancer
目的:探讨过表达羧酸酯酶(carboxylesterases,CES)的人骨髓间充质干细胞(human bone mesenchymal stro-mal cells,hBMSCs)联合LY2334737对裸鼠膀胱癌治疗的影响.方法:将腺病毒介导过表达的人CES2转染hBMSCs,分为空白组(不作任何处理)、空载体组(转染空载体)和转染组(转染过表达CES2腺病毒),流式细胞仪检测转染效率,荧光显微镜观察CES2的表达,Western blot及…查看全部>>
Objective:To investigate the effect of human bone mesenchymal stromal cells(hBMSCs)overexpressing car-boxylesterases(CES)combined with LY2334737 on the treatment of bladder cancer in nude mice.Method:Adenovirus-me-diated overexpressed human CES2 transfected hBMSCs were assigned to the blank group(without any treatment),the empty carrier group(transfected with empty carrier)and the transfection group(transfected with CES2 overexpressed adenovirus).The transfe…查看全部>>
李杨东;范毛川;李卫胜;窦启锋
453199 河南新乡,新乡医学院第一附属医院泌尿外科||550200 贵阳,贵州中医药大学时珍学院护理学部453199 河南新乡,新乡医学院第一附属医院泌尿外科571199 郑州,河南中医药大学第一附属医院泌尿外科453199 河南新乡,新乡医学院第一附属医院泌尿外科
临床医学
膀胱癌人骨髓间充质干细胞羧酸酯酶2LY2334737凋亡增殖
Bladder cancerHuman bone marrow mesenchymal stem cellsCarboxylesterase 2LY2334737Apopto-sisProliferation
《肿瘤预防与治疗》 2024 (10)
831-842,12
河南省科技攻关项目(编号:232102310349)新乡市灾后重建科技专项项目(编号:21CJ002) This study was supported by grants from Science and Technology Department of Henan Province(No.232102310349)and Xinxiang Science and Technology Bureau(No.21CJ002).
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