中国临床药理学杂志2024，Vol.40Issue(20)：2993-2997,5.DOI:10.13699/j.cnki.1001-6821.2024.20.017
11-羰基-β-乙酰乳香酸抑制口腔鳞状细胞癌的研究
Study of acetyl-11-keto-3-boswellic acid inhibiting oral squamous cell carcinoma
摘要
Abstract
Objective To investigate the mechanism of apoptosis induced by acetyl-11-keto-3-boswellic acid(AKBA)in oral squamous cell carcinoma(OSCC)cells.Methods CAL27 were randomly divided into control group(conventional culture),low-dose group(40.00 μmol·L-1 AKBA),middle-dose group(80.00 μmol·L-1 AKBA),high-dose group(120.00 μmol·L-1 AKBA),3-methyladenine(3-MA)group(120.00 μmol·L-1 AKBA+2 mmol·L-1 autophagy inhibitor 3-MA).5-ethynyl-2'-deoxyuridine(Edu)assay was used to detect cell proliferation;Western blot assay was used to detect protein expression;flow cytometry was used to detect apoptosis.Mice were randomly divided into model group(construct OSCC mouse model),AKBA-L group(10.00 mg·kg-1 AKBA after modeling),AKBA-H group(20.00 mg·kg-1 AKBA after modeling),10 animals per group.After 28 days of continuous administration,weight were detected;and the expression of related proteins were detected by Western blot assay.Results The Edu positive cell rates in control group,high-dose group were(40.18±2.53)％,(12.08±0.93)％,respectively;the protein levels of autophagy associated microtubule associated protein 1 light chain 3(LC3)Ⅱ/LC3 Ⅰ in control group,high-dose group and 3-MA group were 0.33±0.05,2.93±0.39,0.56±0.07,respectively;phosphorylated adenylate activated protein kinase catalytic subunit alpha subunit 1(p-PRKAA1)protein levels were 0.34±0.04,1.03±0.07,0.99±0.09,respectively;the apoptosis rates were(4.65±0.39)％,(25.75±2.29)％,(14.92±1.49)％,respectively.The above indexes in hige-dose group were significantly different from those in the control group(all P＜0.05).The above indexes in 3-MA group were significantly different from those in high-dose group(all P＜0.05).The tumor weight of model group,AKBA-L group and AKBA-H group were(0.96±0.08),(0.55±0.06),(0.43±0.05)g,respectively;the protein levels of LC3 Ⅱ/LC3 Ⅰ were 0.47±0.09,0.94±0.21 and 1.69±0.34,respectively.The above indexes in AKBA-L group and AKBA-H group were significantly different from those in model group(all P＜0.05).Conclusion AKBA can induce cytotoxic autophagy related apoptosis and inhibit CAL27 cell proliferation,which may be related to activation of AMPK signal.关键词
11-羰基-β-乙酰乳香酸/口腔鳞状细胞癌/自噬/凋亡/腺苷酸活化蛋白激酶信号通路Key words
acetyl-11-keto-3-boswellic acid/oral squamous cell carcinoma/autophagy/apoptosis/adenylate activated protein kinas signaling pathway分类
医药卫生引用本文复制引用
黄新帮,王羽,俞倩,李勇,李标东,何海蕾,陈琨,赵玲帆..11-羰基-β-乙酰乳香酸抑制口腔鳞状细胞癌的研究[J].中国临床药理学杂志,2024,40(20):2993-2997,5.基金项目
江西省卫生健康委科技计划基金资助项目(SKJP220236690) （SKJP220236690）
