中国临床药理学杂志2024，Vol.40Issue(20)：2998-3002,5.DOI:10.13699/j.cnki.1001-6821.2024.20.018
银杏双黄酮抑制PIK3CA突变驱动的淋巴管畸形的研究
Study on the inhibition of ginkgo biloba flavonoids on PIK3CA mutation-driven lymphatic malformations
陈瑜 1陈慧敏 2王立丹 3刘新献3
作者信息
- 1. 华中科技大学同济医学院附属武汉儿童医院(武汉市妇幼保健院)放射影像科/武汉市儿童影像医学临床医学研究中心,湖北武汉 430000
- 2. 武汉市中医医院超声影像科,湖北武汉 430000
- 3. 华中科技大学同济医学院附属武汉儿童医院(武汉市妇幼保健院)介入放射科/血管瘤科,湖北武汉 430016
- 折叠
摘要
Abstract
Objective To investigate the effect of ginkgo biloba flavonoids on children with lymphatic malformation(LM)driven by phosphatidylinoinosiol 3-kinase(PIK3CA)mutation and its mechanism.Methods The experiment was divided into human dermal lymphatic endothelial cell(HD-LEC)group(no treatment),lymphatic malformation lymphatic endothelial cell(LM-LEC)group(no treatment),ginkgo biflavone group(7.5 μmol·L-1 ginkgo biloba treated LM-LEC cells)and vascular endothelial growth factor-C(VEGF-C)inhibitor group(Sozinibercept group,treated LM-LEC cells with 1 μmol·L-1 VEGF-C inhibitor).The number of lumen and tube branches were observed in each group.Quantitative real-time polymerase chain reaction(qRT-qPCR)was used to detect the relative expression level of VEGF-C.The relative protein expression levels of vascular endothelial growth factor receptor 3(VEGFR3),neurofibrin-2(NRP2),protein kinase B(Akt)and extracellular regulatory protein kinase(ERK)were detected by Western blot.The positive expressions of VEGFR3 and NRP2 were detected by immunofluorescence assay.Results The relative expression levels of VEGF-C mRNA in LM-LEC group and HD-LEC group were 5.42±1.09 and 1.00±0.08;the relative expression levels of VEGFR3 protein were 2.79±0.54 and 1.00±0.10;the relative expression levels of NRP2 protein were 2.58±0.47 and 1.00±0.07;the phosphorylation levels of Akt S473 were 5.62±0.84 and 1.00±0.01;the phosphorylation levels of ERK were 2.37±0.62 and 1.00±0.05,respectively.The positive expressions of VEGFR3 in ginkgo diflavone group,Sozinibercept group and LM-LEC group were 0.35±0.08,0.29±0.08 and 1.00±0.16;the positive expressions of NRP2 were 0.33±0.07,0.31±0.05 and 1.00±0.09,respectively.The above indexes in LM-LEC group were significantly different from those in HD-LEC group,and the above indexes in ginkgo biloba flavonoids group and Sozinibercept group were significantly different from those in LM-LEC group(all P＜0.05).Conclusion Ginkgo biflavone may inhibit the expression of VEGF-C by blocking the VEGF-C/VEGFR-3 signaling pathway,and thus inhibit the lymphatic malformation caused by PIK3CA H1047R mutation.关键词
银杏双黄酮/磷脂酰肌醇3-激酶突变/淋巴管畸形/血管内皮生长因子3/神经纤维蛋白-2Key words
Cinkgo biloba flavonoids/phosphoinositide 3-kinase mutation/lymphatic malformation/vascular endothelial growth factor 3/neurofilament-2分类
中医学引用本文复制引用
陈瑜,陈慧敏,王立丹,刘新献..银杏双黄酮抑制PIK3CA突变驱动的淋巴管畸形的研究[J].中国临床药理学杂志,2024,40(20):2998-3002,5.
