中国临床药理学杂志2024，Vol.40Issue(20)：3003-3007,5.DOI:10.13699/j.cnki.1001-6821.2024.20.019
金丝桃苷对慢性阻塞性肺疾病大鼠骨质疏松的骨代谢、生物力学的影响
Effects of hypericin on bone metabolism,biomechanics and mechanism of osteoporosis in rats with chronic obstructive pulmonary disease
摘要
Abstract
Objective To investigate the effect and mechanism of hypericin on osteoporosis(OP)in rats with chronic obstructive pulmonary disease(COPD).Methods COPD combined with OP rat model was established by cigarette combined with bacteria.The rats were randomly divided into control group,model group(COPD combined with OP model was constructed),experimental-L group(50 mg·kg-1 hypericin was given by intragastric administration after constructing COPD combined with OP model),experimental-H group(100 mg·kg-1 hypericin was given intragastric administration after constructing COPD combined with OP model),positive group(subcutaneous injection of 16 U·kg-1 salmon calcitonin after constructing COPD combined with OP model);each group was given 12 rats for 90 days.The lung function of rats was detected by pulmonary function apparatus;bone mineral density(BMD)was detected by micro-computed tomography(CT);serum bone metabolism and inflammatory factors were detected by enzyme-linked immunosorbent assay(ELISA);Western blot assay was used to detect the relevant indicators of the pathway.Results The levels of forced vital capacity(FVC)in control group,model group,experimental-H group and positive group were(10.42±1.40),(4.10±0.60),(6.75±0.37),(4.18±0.33)mL,respectively;BMD levels were(0.31±0.04),(0.12±0.02),(0.28±0.03),(0.29±0.04)g·mm-3,respectively;bone alkaline phosphatase(BALP)levels were(200.04±20.03),(80.80±6.00),(148.16±14.23),(173.97±23.55)U·L1,respectively;interleukin-1β(IL-1β)levels were(122.60±8.70),(695.59±74.84),(422.41±44.86),(527.90±39.36)pg·mL-1,respectively;phosphorylated p38 mitogen-activated protein kinase(p-P38)protein expression levels were 0.99±0.11,0.36±0.05,0.79±0.08,0.36±0.04,respectively.Compared with the control group,the above indexes in the model group had statistical significance(all P＜0.05);the above indexes in experimental-H group were significantly different from those in model group(all P＜0.05).Conclusion Hypericin can inhibit inflammatory response,improve bone metabolism and biomechanics.关键词
金丝桃苷/慢性阻塞性肺疾病/骨质疏松/骨代谢/炎性因子/p38丝裂原活化蛋白激酶信号通路Key words
hypericin/chronic obstructive pulmonary disease/osteoporosis/bone metabolism/inflammatory factors/p38 mitogen-activated protein kinase signaling pathway分类
医药卫生引用本文复制引用
沈明霞,丁文君,谢海彬,张耘,杨星星,高永蕊..金丝桃苷对慢性阻塞性肺疾病大鼠骨质疏松的骨代谢、生物力学的影响[J].中国临床药理学杂志,2024,40(20):3003-3007,5.基金项目
甘肃省自然科学基金资助项目(20JR10RA342、22JR5RA642) （20JR10RA342、22JR5RA642）
甘肃中医药大学附属医院院内创新基金资助项目(Gzfy-2022-05) （Gzfy-2022-05）
