中国临床药理学杂志2024，Vol.40Issue(20)：3038-3041,4.DOI:10.13699/j.cnki.1001-6821.2024.20.026
用UHPLC-MS/MS法测定比格犬血浆中拉西地平的浓度
Determination of lacidipine in Beagle dog plasma by UHPLC-MS/MS method
摘要
Abstract
Objective To establish an ultra high performance liquid chromatography-tandem mass spectrometry method for the determination of lacidipine in plasma of beagle dogs was established.Methods It was pretreated by protein precipitation method and the internal standard was nimodipine.Chromatographic column:ACQUITYUPLC® BEH C8(2.1 mm x50.0 mm,1.7 μm),mobile phase:100％water containing 5 mmol·L-1 ammonium acetate-100％acetonitrile,flow rate:0.7 mL·min-1,column temperature:40 ℃,automatic injector temperature:4 ℃,injection volume:20 μL.Electrospray ionization source,positive ion mode,multi-reaction monitoring.The specificity,residual effect,standard curve and quantitative lower limit,precision and recovery,matrix effect and stability of the method were investigated.Results Lacidipine has a good linear relationship in the range of 0.10-50.0 ng·mL-1,r=0.996 6,the lower limit of quantification was 0.10 ng·mL-1.The specificity was good.The intra-and inter-relative standard deviation was less than 12％.The extraction recovery was higher than 80％,and the stability was good.Conclusion The method has the advantages of high sensitivity,simple operation and short analysis time,and was suitable for the pharmacokinetic study of lacidipine in Beagle dog plasma.关键词
拉西地平/超高效液相色谱-串联质谱法/血药浓度Key words
lacidipine/ultra-high performance liquid chromatography-tandem mass spectrometry/drug plasma concentration分类
医药卫生引用本文复制引用
王燕燕,姜宜宏,赵小英,周真宇,李晓红,阎成炟,秦峰..用UHPLC-MS/MS法测定比格犬血浆中拉西地平的浓度[J].中国临床药理学杂志,2024,40(20):3038-3041,4.基金项目
沈阳药科大学中青年教师事业发展支持计划基金资助项目(ZQN202305) （ZQN202305）
