北京中医药大学学报2024，Vol.47Issue(10)：1466-1473,8.DOI:10.3969/j.issn.1006-2157.2024.10.017
电针对帕金森病小鼠肠道NEK7/NLRP3炎症信号通路的影响
Effect of electroacupuncture on the NEK7/NLRP3 inflammatory signaling pathway in intestine of mice with Parkinson's disease
摘要
Abstract
Objective To investigate the effects of electroacupuncture on the intestinal NIMA-related kinase 7(NEK7)/NOD-like receptor thermal protein domain associated protein 3(NLRP3)signaling pathway in mice with Parkinson's disease.Methods According to the randomized number table method,36 C57BL/6 mice were randomly divided into the control group,the model group,and the electroacupuncture group,with 12 mice per group.The Parkinson's disease mouse model was established by gavage of rotenone solution(10 mg/kg)for 28 d.After molding,the electroacupuncture group was stimulated with"Fengfu"(DU17),"Taichong"(LR3),and"Zusanli"(ST36)for 14 d,while the control group and the model group were only treated with immobilization.The motor ability of mice was detected by pole climbing test and hindlimb rating score,the positive expressions of nigral tyrosine hydroxylase(TH)and colon occludin were detected by immunohistochemistry,the histological morphology of colon was observed by hematoxylin-eosin staining,and Western blotting was used to detect the protein expressions of NEK7,NLRP3,Caspase-1,and interleukin-1β(IL-1β).Results Compared with the control group,mice in the model group had lower score on the pole climbing test and a higher hindlimb rating score(P＜0.01);the average optical densities of nigral TH and colon occludin were decreased(P＜0.01);significant inflammatory infiltration was observed in the colonic tissue,and the muscularis propria was thinned;and the protein expressions of NEK7,NLRP3,Caspase-1,and IL-1β in the colonic tissue were elevated(P＜0.01).Compared with the model group,mice in the electroacupuncture group had higher score on the pole climbing test and a lower hindlimb rating score(P＜0.01);the average optical densities of nigral TH and colon occludin were increased(P＜0.01);the degree of inflammatory infiltration of colonic tissues decreased,and the muscularis propria was thickened;and the protein expressions of NEK7,NLRP3,Caspase-1,and IL-1β of colonic tissues were decreased(P＜0.01).Conclusion Electroacupuncture at"Fengfu"(DU17),"Taichong"(LR3),and"Zusanli"(ST36)can improve motor functional impairments in mice with Parkinson's disease,and the mechanism may be through the inhibition of intestinal NEK7/NLRP3 pathway,improving the intestinal barrier damage,relieving the intestinal inflammation,and improving the dopaminergic neuron injury.关键词
帕金森病/电针/肠道炎症/NIMA相关激酶7/NOD样受体热蛋白结构域相关蛋白3/小鼠Key words
Parkinson's disease/electroacupuncture/intestinal inflammation/NIMA-related kinase 7/NOD-like receptor thermal protein domain associated protein 3/mice分类
医药卫生引用本文复制引用
李含章,李亚楠,郭磊,汪瑶,张小蕾,胡梦妮,祁羚,荣臻,马骏..电针对帕金森病小鼠肠道NEK7/NLRP3炎症信号通路的影响[J].北京中医药大学学报,2024,47(10):1466-1473,8.基金项目
中国博士后科学基金项目(No.2023M741110) （No.2023M741110）
国家自然科学基金项目(No.81473788) Postdoctoral Science Foundation of China(No.2023M741110) （No.81473788）
