农业生物技术学报2024，Vol.32Issue(11)：2447-2461,15.DOI:10.3969/j.issn.1674-7968.2024.11.001
水稻粒型全基因组关联分析
Genome-wide Association Analysis of Rice(Oryza sativa)Grain Shape
摘要
Abstract
Rice(Oryza sativa)grain shape is a critical factor in determining the yield,appearance,nutritional quality,and commercial value of rice.Grain shape is primarily determined by genetic factors.It is crucial to enhance the quality and yield of rice by exploring and utilizing valuable gene resources related to rice grain shape,as well as analyzing the molecular mechanisms that regulate it.In this study,410 3K rice core germplasms were used as research materials.After seed maturation,measurements were taken for grain length,grain width,and grain thickness.Genome-wide association analysis(GWAS)was conducted using the genotypes of the germplasm.Candidate genes and haplotypes were analyzed to screen for potential genes associated with these QTL.A total of 5 QTL related to grain shape were detected by GWAS,which were distributed on chromosomes 1,3,5,7,and 8 of rice.The QTL controlling grain length were qGL3,qGL7,and qGL8.Among these,qGL3 was co-located with GS3,qGL7 was co-located with GL7,and qGL8 was a newly discovered QTL locus that controls grain length.The QTL controlling grain width was qGW5,which was co-located with GW5.The QTL controlling grain thickness was qGT1,which was co-located with d61.The candidate gene for qGL8 was Os08g0421800.The analysis involved aggregating different haplotypes of grain length QTL in germplasm.It was found that the favorable haplotypes of the candidate genes qGL3,qGL7,and qGL8 were present in 8 germplasms,including'B073'and'B081'.Additionally,the grains of these 8 germplasms were slender.This study provides new germplasm resources and theoretical support for enhancing the genetic improvement of rice grain shape.关键词
粒型/QTL/全基因组关联分析(GWAS)/单倍型/聚合Key words
Grain Shape/QTL/Genome-wide association analysis(GWAS)/Haplotype/Polymerization分类
农业科技引用本文复制引用
龚成云,朱裕敬,桂金鑫,石居斌,罗新阳,曾哲,张海清,贺记外..水稻粒型全基因组关联分析[J].农业生物技术学报,2024,32(11):2447-2461,15.基金项目
湖南省自然科学基金(2020JJ5232) （2020JJ5232）
