农业生物技术学报2024，Vol.32Issue(11)：2462-2473,12.DOI:10.3969/j.issn.1674-7968.2024.11.002
小麦WUS基因亚家族的全基因组鉴定及其在雄蕊和雌蕊中的表达分析
Genome-wide Identification and Expression Analysis of WUS Gene Subfamily in Wheat(Triticum aestivum)Pistil and Stamen
摘要
Abstract
WUSCHEL(WUS)is a homeodomain transcription factor,an evolutionary branch of the WOX family,which may play an important role in embryogenesis and bud regeneration in plant cells by regulating the expression of specific target genes.In this study,a total of 25 WUS genes were identified in the entire genome of wheat(Triticum aestivum)by bioinformatics methods.These genes were distributed unevenly on the first,second,third and the fifth homologous group of chromosomes,with tandem duplication observed on the third homologous group chromosomes.Conserved domain and structural analysis showed that motif 1 and motif 3 were present in all members of the family,and the WUS proteins clustered together showed similar gene structure according to the evolutionary tree.A total of 18 hormone response and abiotic stress response elements were identified in the promoter region of TaWUSs.Tissue expression profile analysis showed that the 25 detected TaWUSs had obvious tissue expression specificity in wheat,and most of them showed high expression in the floral reproductive organs.qRT-PCR results also confirmed that TaWUS-2A1,TaWUS-3B2,TaWUS-3D2,TaWUS-5A1,TaWUS-5B1 and TaWUS-5D1 were highly expressed in stamens,and TaWUS-3D1 was highly expressed in pistils.Especially,the high expression of TaWUS-1A,TaWUS-1B,TaWUS-1D,and TaWUS-2D1 in pistillody stamens might indicate their involvement in regulating the formation of stamen homologous transformation into pistil traits in the wheat homologous transformation sterility-1(HTS-1).This study provides a theoretical basis for further research on the function of wheat WUS subfamily genes in wheat.关键词
小麦/WUS基因亚家族/雄蕊/雌蕊Key words
Triticum aestivum/WUS gene subfamily/Stamen/Pistil分类
农业科技引用本文复制引用
赵若男,邹瑞,YAMAMOTO Naoki,陈真勇,吴一超,蒋进,彭正松,杨在君..小麦WUS基因亚家族的全基因组鉴定及其在雄蕊和雌蕊中的表达分析[J].农业生物技术学报,2024,32(11):2462-2473,12.基金项目
国家自然科学基金(32272066 （）
32060456) （）
四川省自然科学基金(2022NSFSC0160) （2022NSFSC0160）
四川省科学技术厅重大科技专项子课题(2022ZDZX0014-4-4) （2022ZDZX0014-4-4）
