农业生物技术学报2024，Vol.32Issue(11)：2484-2497,14.DOI:10.3969/j.issn.1674-7968.2024.11.004
StWIP2基因调控马铃薯对晚疫病的抗性
StWIP2 Regulates the Resistance to Phytophthora infestans in Potato(Solanum tuberosum)
摘要
Abstract
Wound-induced proteins(WIPs)play an essential role in plants defence responses to pathogen infestation.In this study,the StWIP2 gene(GenBank No.NM_001288699.1)was cloned from potato(Solanum tuberosum)'Qingshu 9',and bioinformatics analyses of the StWIP2 gene were carried out,while overexpression vectors and silencing vectors were constructed,and reverse genetics was used to characterise the function of the StWIP2 gene in the interaction with Phytophthora infestans.Bioinformatics revealed that the StWIP2 gene had a full CDS length of 636 bp and encoded a total of 211 amino acids.qPCR analysis revealed that the StWIP2 gene had the highest expression in'Chunshu 5'and the lowest expression in diploid potato DM.The StWIP2 gene was responsive and differentially expressed under treatments of P.infestans,salicylic acid(SA),abscisic acid(ABA),and methyl jasmonate(MeJA).Overexpression of StWIP2 was able to increase the content of H2O2 and malondialdehyde(MDA),the activity of antioxidant enzymes,and the expression levels of pathogenesis related protein 1(StPR1),StPR5 and ethylene responsive factor 1(StERF1),to delay the onset of the disease in the plants and to increase the level of plant resistance to P.infestans.After silencing the StWIP2 gene,the content of H2O2 and MDA in plants decreased,and the activity of antioxidant enzymes decreased;The expression levels of plant defense gene 1.2(PDF 1.2),StERF1,peroxidase gene(StPOD),and phenylalanine ammonia lyase(StPAL)were reduced,while the expression of StPR1 and StPR5 were inhibited,resulting in a decrease in plant resistance to late blight.The above results indicate that the StWIP2 gene plays a positive regulatory role in the interaction with P.infestans,and the StWIP2 gene can be utilized as a candidate genetic resource for disease resistance breeding in potatoes.关键词
马铃薯/致病疫霉/伤害诱导蛋白基因(StWIP2)/抗病性鉴定/抗氧化酶Key words
Potato/Phytophthora infestans/Wound-induced proteins(SStWIP2)/Disease resistance identification/Antioxidant enzymes分类
农业科技引用本文复制引用
朱迪,周云,杨生龙,孙海宏,贺苗苗..StWIP2基因调控马铃薯对晚疫病的抗性[J].农业生物技术学报,2024,32(11):2484-2497,14.基金项目
青海省科技厅基础研究计划项目(2022-ZJ-712) （2022-ZJ-712）
国家马铃薯产业技术体系(CARS-09) （CARS-09）
