农业生物技术学报2024，Vol.32Issue(11)：2552-2563,12.DOI:10.3969/j.issn.1674-7968.2024.11.009
牛卵泡颗粒细胞中Wnt/β-catenin与Notch信号通路关键因子的表达规律
Expression Patterns of Key Factors of Wnt/β-catenin and Notch Signaling Pathway in Bovine(Bos taurus)Follicular Granulosa Cells
摘要
Abstract
The proliferation,apoptosis and steroid hormone synthesis of granulosa cells play important roles in follicle development,and these functions are regulated by signaling pathways.In this study,the transcriptome sequencing of granulosa cells from the first large follicle F1 and the second large follicle F2 in bovine(Bos taurus)first follicle wave pre-deviation(PD)and onset of diameter deviation(OD)were separately performed to obtain the expression patterns of genes in Wnt/β-catenin and Notch signaling pathways,cell cycle,apoptosis and steroid hormone synthesis,and to validate the expression of key components of the 2 pathways.The results showed that the genes of key components of the 2 pathways with high expression in PDF1,PDF2,ODF1 and ODF2 follicular granulosa cells were wingless-type MMTV integration site family,member 10a(Wnt10a),Wnt2b,frizzled3(Fzd3),low density lipoprotein-related receptor 8(LRP8),dishevelled 3(Dvl3),Axin1,glycogen synthase 3β(GSK3β),catenin beta 1(CTNNB1),transcription factor 7-like 2(TCF7L2),lymphoid enhancer factor 1(LEF1),B-cell CLL/Lymphoma 9(BCL9),Notch2,Dll4 and Hes related with YRPW motif2(Hey2).Cell cycle-related genes with high expression were cyclin2(CCND2),cyclindependent kinase4(CDK4),cyclin-dependent kinase inhibitor 1A(CDKN1A),CDKN1B.The apoptosis of granulosa cells was mainly regulated by BCL2-associated X(Bax)in the mitochondrial apoptosis pathway and executed by Caspase3 and Caspase6.The expression of the steroid genes were 3β-hydroxysteroid dehydrogenase(3β-HSD),cytochrome P450 family 11 subfamily A member 1(CYP11A1),and CYP19A1 were elevated,and E2 synthesis ability of OD follicular granulosa cells was increased compared with that of PD follicles.Wnt2 protein was localized in follicular granulosa cells in deviation process and its expression was significantly higher in OD follicular granulosa cells(P＜0.05);Wnt2b protein was localized in follicular granulosa cells and theca cells and its expression was extremely significant lower in OD follicular granulosa cells(P＜0.01).The mRNA expression of CTNNB1,LEF1,Notch2 and Hey2 were all extremely significantly increased in OD follicular granulosa cells(P＜0.01).This study showed that the gene expression in Fl and F2 follicular granulosa cells during the deviation of bovine first follicular wave was specific,and the gene expression of key components in Wnt/β-catenin and Notch signaling pathway had the same trend,which provides a reference basis for the molecular regulatory mechanism of granulosa cell function during follicular development.关键词
牛卵泡颗粒细胞/Wnt、Notch信号通路/细胞周期/凋亡/类固醇激素Key words
Bovine follicular granulosa cells/Wnt and Notch signaling pathway/Cell cycle/Apoptosis/Steroid hormone分类
农业科技引用本文复制引用
党文庆,何敏,曲玉涵,张凯,杨小峰,秦小伟,韩琦,郭翔宇,吕丽华..牛卵泡颗粒细胞中Wnt/β-catenin与Notch信号通路关键因子的表达规律[J].农业生物技术学报,2024,32(11):2552-2563,12.基金项目
山西省基础研究计划项目(面上项目,20210302123393) （面上项目,20210302123393）
山西省大同市现代产业研究技术体系项目(农业,2021027) （农业,2021027）
山西省基础研究计划项目(202303021221095) （202303021221095）
山西农业大学"引进人才科研启动工程"项目(2023BQ56) （2023BQ56）
