农业生物技术学报2024，Vol.32Issue(11)：2627-2639,13.DOI:10.3969/j.issn.1674-7968.2024.11.016
生防菌株贝莱斯芽胞杆菌ZF513的分离鉴定及其对黄瓜猝倒病的防治效果
Isolation,Identification and Control Effect of Bacillus velezensis ZF513 Against Damping-off of Cucumber(Cucumis sativus)
摘要
Abstract
Damping-off of cucumber caused by Pythium aphanidermatum is a serious seedling disease in cucumber(Cucumis sativus)production,which seriously affects the yield and quality of cucumber.In order to obtain a biocontrol strain with antagonistic effect against damping-off of cucumber,in this study,Bacillus spp.were isolated from the inter-root soil of healthy cucumber using a gradient dilution plating method,and obtained the biocontrol strain ZF513 with the best preventive effect against damping-off of cucumber,which amounted to 86.64％,through potted plants in greenhouse.After morphological,physiological and biochemical tests,unique carbon source utilization determination and molecular identification,it was determined that the biocontrol strain ZF513 was Bacillus velezensis.Plate confrontation method was used to determine that strain ZF513 had obvious antagonistic effect on 8 kinds of plant pathogens(Pythium aphanidermatum,Rhizoctonia solani,Fusarium oxysporum,F.solani,Corynespora cassiicola,Phytophthora capsici,Botrytis cinerea,Mphylium solani)with a broad-spectrum bacterial inhibition ability,in which the inhibition of P.aphanidermatum reached 65.23％.The results of pot test showed that strain ZF513 had obvious growth-promoting effect on cucumber plants,and the highest prevention effect on damping-off of cucumber was 72.61％when the concentration was 1×108 cfu/mL and the dosage was 10 mL/plant,and the strain could stabilize and colonize the inter-root soil of cucumber with the concentration of 105 cfu/g,which could protect the cucumber plants from disease invasion during the period of seedlings.In conclusion,strain ZF513 has potential application value in the control of damping-off of cucumber.关键词
贝莱斯芽胞杆菌/瓜果腐霉/黄瓜猝倒病/生物防治/促生作用Key words
Bacillus velezensis/Pythium aphanidermatum/Damping-off of cucumber/Biocontrol/Growth promotion分类
农业科技引用本文复制引用
闫丽霞,李磊,丁常宗,石延霞,柴阿丽,范腾飞,谢学文,李宝聚..生防菌株贝莱斯芽胞杆菌ZF513的分离鉴定及其对黄瓜猝倒病的防治效果[J].农业生物技术学报,2024,32(11):2627-2639,13.基金项目
财政部和农业农村部国家现代农业产业技术体系(CARS-23) （CARS-23）
中国农业科学院科技创新工程项目(CAAS-ASTIP-IVFCAAS) （CAAS-ASTIP-IVFCAAS）
农业农村部园艺作物生物学与种质创制重点实验室(IVF2023) （IVF2023）
蔬菜生物育种国家重点实验室开放课题 （）
