皮革科学与工程2024，Vol.34Issue(6)：24-30,7.DOI:10.19677/j.issn.1004-7964.2024.06.004
基于低共熔溶剂的牛皮胶原提取工艺研究
Extraction of Collagen from Cowhide by Deep Eutectic Solvent Aqueous Solutions
摘要
Abstract
Deep eutectic solvent(DES)aqueous solutions were utilized to extract collagen from deliming cowhide trimmings.The effect of DES type,DES concentration,extraction time and temperature on the collagen extraction efficiency were investigated.Results showed that the optimal extraction efficiency can be obtained by using the DES containing urea and acetic acid as the extraction agent,with the DES concentration of 0.75 mol/L,temperature of 15 ℃ and extraction time of 24 h,respectively.Under this condition,the yield of collagen was 37％;the results of infrared spectroscopy showed that the product held a typical absorption peak of type Ⅰ collagen.Compared with the collagen extracted by traditional acidic and enzymatic methods,the collagen extracted using DES aqueous solution displayed higher purity of collagen and intact triple helix structure,which endow it with wide potential in biomedical field.关键词
低共熔溶剂/胶原/提取工艺/牛皮Key words
deep eutectic solvents/collagen/extraction process/cowhide分类
轻工纺织引用本文复制引用
俞小凤,宋文祥,许波,刘颖,王平..基于低共熔溶剂的牛皮胶原提取工艺研究[J].皮革科学与工程,2024,34(6):24-30,7.基金项目
国家自然科学基金青年基金项目(22308122) （22308122）
