西南林业大学学报2024，Vol.44Issue(9)：112-119,8.DOI:10.11929/j.swfu.202311058
绿孔雀肠道菌群基因组特征和耐药性的宏基因组测序分析
Genomic Characteristics and Drug Resistance of Intestinal Flora of Pavo muticus were Analyzed by Metagenomic Sequencing
摘要
Abstract
To clarify the distribution characteristics of drug resistance genes and mobile elements in intestinal flora of Pavo muticus,the feces of healthy Pavo muticus raised in Yunnan Wildlife Park were sampled,and the DNA was extracted for metagenomic sequencing.Species annotation and relative abundance calculation were car-ried out using NCBI-nr database to obtain the species distribution characteristics of intestinal flora of P.muticus.Through functional annotation and cluster analysis,the types and distribution characteristics of drug resistance genes were obtained.The BLAST program was used to search for mobile resistance genes in samples,and the bacterial genomes in different regions,different hosts and different body positions were compared to analyze their differences and the frequency of recent horizontal transfer.At the same time,flanking regions of each mobile Anatibiotic resistance gene(ARGs)were searched for analysis in order to identify mobile genetic elements(MGEs)associated with ARGs.The results showed that the intestinal flora species of P.muticus have strong bias,Prevotella is the dominant species,bacteroidetes is the dominant bacteria.The mobile resistance genes carried by bacteria were mainly ant(6)-Ⅰa,erm(B)and tet(W/32/O).The abundance of aph(3")-Ⅰb genes in the samples was low,but the horizontal transfer frequency was high,because the flanking region was rich in a large number of MGEs.The acquisition of drug resistance genes in the samples mainly depended on the assistance of IS5 transpo-sons.The distribution of drug resistance genes is biased,mainly concentrated in Proteobacteria and Firmicutes,and at the species level,Streptococcus suis and Escherichia coli carry more drug resistance genes and are highly mobile.The horizontal transfer of drug resistance genes is easily affected by antibiotic use strategy,drug resist-ance gene species,ecological environment and genetic factors of host phylogeny.For P.muticus in Yunnan Wild-life Park,aminoglycoside resistance gene monitoring should be strengthened in clinic,such antibiotics should be avoided,and the infection of Streptococcus suis and Escherichia coli should be vigilant.关键词
绿孔雀/肠道菌群/耐药性/基因/可移动元件Key words
Pavo muticus/intestinal flora/drug resistance/gene/mobile element分类
畜牧业引用本文复制引用
苗睿,刘垒成,姜玉莎,赵浪,孙嘉良,吴培福..绿孔雀肠道菌群基因组特征和耐药性的宏基因组测序分析[J].西南林业大学学报,2024,44(9):112-119,8.基金项目
云南省教育厅科学研究基金项目(2022J0505)资助 （2022J0505）
生物学质量工程项目(503190106)资助. （503190106）
