西南林业大学学报2024，Vol.44Issue(9)：120-128,9.DOI:10.11929/j.swfu.202311026
2种樟树叶片基质添加对牛樟芝三萜含量及抗氧化活性影响
Effects of 2 Camphor Tree Leaf Substrates on the Triterpene Contents and Antioxidant Activities of Antrodia cinnamomea
摘要
Abstract
Compare and analyze the effects of adding 2 different concentrations of camphor tree leaf homo-genate substrates to rice culture medium on the triterpenoid content and antioxidant activity of Antrodia cinnamo-mea,select the most suitable culture conditions,and provide reference for the artificial cultivation of A.cinnamo-mea and the development and utilization.Using rice culture medium as a control treatment(CK),a total of 6 con-centrations of 0(CK),0.5,1,2,3,and 4 g of Cinnamomum kanehirai and Cinnamomum camphora leaf homogen-ate matrices were added.The growth characteristics and triterpene contcentration of A.cinnamomea mycelia were measured under the 6 different treatments,and their free radical scavenging and total reducing abilities against 1,1-diphenyl-2-trinitrylhydrazine(DPPH)were compared to evaluate the antioxidant activity of A.cinnamomea.The results showed that adding different concentrations of C.kanehirai and C.camphora leaf homogenate matrix significantly improved the growth and biological activity of A.cinnamomea,and each treatment completed peak growth from 9 to 27 days.After comprehensive evaluation of growth characteristics factors,added 3 g of C.cam-phora substrate to rice culture medium significantly promoted the growth of A.cinnamomea,which was 4 times higher than the CK,which was the optimal treatment to promote the growth of A.cinnamomea;addition 1 g of C.kanehirai matrix significantly increased the triterpene contcentration,DPPH scavenging ability,and total reduc-tion ability of A.cinnamomea,which were 5.2,15.8,and 23 times higher than the CK,respectively.This is the op-timal treatment for improving the triterpenoid content and antioxidant activity of A.cinnamomea.关键词
牛樟芝/牛樟/香樟/三萜含量/抗氧化活性Key words
Antrodia cinnamomea/Cinnamomum kanehirai/Cinnamomum camphora/triterpene contcentra-tion/antioxidant activity分类
生物学引用本文复制引用
郑洋群,尹以杭,余思廷,赵晓雷,石晓龙,郑元..2种樟树叶片基质添加对牛樟芝三萜含量及抗氧化活性影响[J].西南林业大学学报,2024,44(9):120-128,9.基金项目
国家自然科学基金项目(32160736)资助 （32160736）
云南省农业基础研究联合专项重点项目(202101BD070001-020)资助 （202101BD070001-020）
云南省基础研究计划面上项目(202101AT070044)资助 （202101AT070044）
"兴滇英才支持计划"青年人才专项项目(XDYC-QN-RC-2022-0245)资助. （XDYC-QN-RC-2022-0245）
