西南林业大学学报2024，Vol.44Issue(9)：207-214,8.DOI:10.11929/j.swfu.202311019
产香云芝菌丝体的培养条件优化及其挥发性成分分析
Optimization of Culture Conditions and Analysis of Volatile Components of a Fragrant Trametes versicolor Mycelium
摘要
Abstract
In order to obtain the optimal culture conditions and volatile components of Trametes versicolor mycelium,a single factor experimental method was used to study the effects of different carbon sources,nitrogen sources,pH values,medium volume,culture temperature,and culture time on the production of volatile compon-ents in T.versicolor mycelium.The culture conditions were further optimized by response surface methodology and the volatile components of T.versicolor mycelium were analyzed by gas chromatography-mass spectrometry.The results showed that the volume of culture medium,culture time,and culture temperature had a significant im-pact on the volatile components of T.versicolor mycelium.The optimal culture conditions were sucrose as the carbon source,no nitrogen source added,120 mL of culture medium volume,27.5 ℃ of culture temperature,and 27 days of culture time.Under these conditions,the error between the validation test results with the theoretical prediction is 0.315％.A total of 6 volatile components were detected from the mycelium of T.versicolor,includ-ing 3 alcohols,1 aromatics,and 2 sesquiterpenes.Among them,1-epibicyclosesquiphellandrene and(E)-cala-menene were the main components,with relative contents of 29.96％and 29.17％,respectively.This provides a basis for the further development and application of T.versicolor mycelium in microbial fragrances.关键词
云芝菌丝体/顶空固相微萃取/响应面法/产香真菌/气相色谱-质谱Key words
Trametes versicolor mycelium/HS-SPME/response surface method/aromatherogenic fungi/GC-MS分类
化学化工引用本文复制引用
吴博宇,鲁斌,尹文行,萧卫泓,王玮,严冬,赵平..产香云芝菌丝体的培养条件优化及其挥发性成分分析[J].西南林业大学学报,2024,44(9):207-214,8.基金项目
国家自然科学基金项目(32060107,32060327)资助. （32060107,32060327）
