中医康复2024，Vol.1Issue(11)：19-24,6.DOI:10.19787/j.issn.2097-3128.2024.11.005
青藤碱对骨关节炎软骨细胞增殖和炎症的影响
Sinomenine Regulates the Effects of Wnt/β-catenin Signaling Pathway on Chondrocyte Proliferation and Inflammatory Factors in Osteoarthritis
摘要
Abstract
Objective:To investigate the effect of Sinomenine on the proliferation and inflammation of OA chondrocytes,and to explore its specific mechanism.Methods:Chondrocytes were induced by 10ng/mL interleukin 1β(IL-1β)to establish a model of osteoarthritis chondrocyte injury,and different concentrations of sinomenine were added to treat the cells.CCK-8 assay was used to detect cell proliferation;ELISA was used to detect the levels of IL-6 and TNF-α;Western blot was used to detect the expression of IL-6,TNF-α,Wnt and β-catenin proteins;The expressions of IL-6,TNF-α,Wnt and β-catenin were detected by qRT-PCR.Results:Different concentrations of sinomenine could reverse the decrease of chondrocyte prolif-eration activity induced by IL-1β,and the difference was statistically significant(P<0.05),but there was no significant difference between the groups(P>0.05).At the same time,the levels of IL-6 and TNF-α inflammatory factors were also downregulated by different concentrations of sinomenine,and the downregulation of low-concentration and high-concentration sinomenine was more significant,and the difference was statistically significant(P<0.05),but there was no significant difference between the low-concentration and high-concentration groups(P>0.05).Low concentration of sino-menine could reduce the protein and gene expression levels of IL-6 and TNF-α in chondrocytes,and also down-regulate the protein and gene expres-sion of Wnt and β-catenin,and the difference was statistically significant(P<0.05).However,the protein and gene expressions of IL-6,TNF-α,Wnt and β-catenin were not significantly reduced at medium and high concentrations,and the difference was not statistically significant(P>0.05).Conclu-sion:Low concentrations of sinomenine may promote chondrocyte proliferation and inhibit inflammation in OA chondrocytes,which may be partly related to the regulation of the Wnt/β-catenin signalling pathway.关键词
骨关节炎/青藤碱/炎症/软骨细胞/Wnt/β-cateninKey words
osteoarthritis/sinomenine/inflammation/chondrocyte/Wnt/β-catenin分类
医药卫生引用本文复制引用
刘结梅,冯杰扬,丁家谊,欧阳凤珊,刘锦灿..青藤碱对骨关节炎软骨细胞增殖和炎症的影响[J].中医康复,2024,1(11):19-24,6.基金项目
广东省中医药局科研项目(20221384) （20221384）
