中医康复2024，Vol.1Issue(12)：44-48,5.DOI:10.19787/j.issn.2097-3128.2024.12.007
"足三里""尺泽"电针预处理对急性肺损伤模型大鼠炎症反应及Nrf-2、HO-1因子表达的影响
The Effect of Electroacupuncture Pretreatment at"Zusanli"(ST 36)and"Chize"(LU 5)Points on the Inflammatory and Nrf-2/HO-1 Pathway in Rats with Sepsis-induced Acute Lung Injury
摘要
Abstract
Objective:To observe the effect of electroacupuncture(EA)pretreatment at"Zusanli"(ST 36)and"Chize"(LU 5)points on the inflamma-tory and erythroid 2-related factor 2/Heme oxygenase 1(Nrf-2/HO-1)pathway in rats with sepsis-induced acute lung injury(ALI),and to explore the protective effect and possible mechanism of EA pretreatment in ALI.Methods:According to the principle of randomization,18 Wistar male rats were equally divided into 3 groups,namely the control group,LPS model group and EA preconditioning+LPS model group.The ALI model was estab-lished by intraperitoneal injection of lipopolysaccharide(LPS).EA(3 Hz/15 Hz,1～2 mA)was applied to bilateral ST 36 and LU 5 for 30 min,once a day for 5 consecutive days before modeling.HE staining was used to observe the pathological changes of the lung,and the light microscopy was ob-served and the pathological score of lung injury was performed;the secretions of inflammatory factors IL-1β and IL-10 in the serum and lung tissue were measured by ELISA kits;and lung tissue Nrf-2/HO-1 pathway-related mRNA and protein expression were detected by PCR and Western Blot.Results:Compared with the control group,the alveolar wall of the model group was thickened,a large number of inflammatory cells were infiltrated in the lung interstitium,and red blood cell exudation was observed in the alveolar;serum IL-1β levels were increased,and IL-10 levels were de-creased(P<0.01).The mRNA expressions of Nrf-2 and HO-1 were decreased(P<0.01).Compared with the model group,the degree of lung injury in the electroacupuncture pretreatment+model group was significantly reduced.The levels of IL-1β were decreased(P<0.01),while the levels of IL-10 were increased(P<0.05).The mRNA and protein expression levels of Nrf2 and HO-1 were significantly increased(P<0.01,P<0.05).The trend of protein expression was consistent with mRNA expression.Conclusions:EA pretreatment can reduce pulmonary inflammatory response in ALI rats,and its protective effect on the lung may be related to inhibiting the expression of Nrf2,promoting the secretion of HO-1 level of pro-inflammatory factors and neutrophil infiltration in the lung.关键词
电针预处理/急性肺损伤/炎症反应/Nrf-2/HO-1/大鼠Key words
electroacupuncture pretreatment/acute lung injury/inflammation/Nrf-2/HO-1/rats分类
医药卫生引用本文复制引用
彭婷,罗敷,谭金曲,舒象忠,周君,宁鹏云,曾亚华,黄夏荣,王婷,屈萌艰.."足三里""尺泽"电针预处理对急性肺损伤模型大鼠炎症反应及Nrf-2、HO-1因子表达的影响[J].中医康复,2024,1(12):44-48,5.基金项目
湖南省自然科学基金青年项目(2023JJ40590) （2023JJ40590）
湖南省自然科学基金青年项目(2023JJ40595) （2023JJ40595）
湖南省卫生健康委员会一般项目(B202314058540) （B202314058540）
