饲料研究2024，Vol.47Issue(19)：80-85,6.DOI:10.13557/j.cnki.issn1002-2813.2024.19.016
地黄总黄酮提取工艺优化及其抗氧化活性研究
Optimization of extraction method of total flavonoids from Rehmannia glutinosa and study on its antioxidant activity
摘要
Abstract
The study aimed to optimize the extraction process of total flavonoids from Rehmannia glutinosa using the response surface methodology and to evaluate the total flavonoid content and antioxidant capacity in the radial striations,no-radial striations and root of Rehmannia germplasm QH-1 and Jinjiu.The results showed that the optimal extraction conditions for total flavonoids from Rehmannia glutinosa were a liquid-to-solid ratio of 30 mL/g,ethanol volume fraction of 50％,extraction temperature of 50 ℃,and extraction time of 40 min,with a total flavonoid extraction rate of 0.130 2％.The content of total flavone of Rehmannia glutinosa in both germplasm was non-radial striations parts＞radial striations parts.The IC50 values for scavenging 1,l-diphenyl-2-picrylhydrazyl(DPPH)free radicals of QH-1 Rehmannia glutinosa radial striations,no-radial striations,and root were 0.016,0.009,and 0.009 g/L,respectively,while those for Jinjiu Rehmannia glutinosa were 0.006,0.009,and 0.010 g/L,respectively.The IC50 values for scavenging hydroxyl free radicals of QH-1 Rehmannia glutinosa radial striations,no-radial striations,and root were 0.054,0.035,and 0.047 g/L,respectively,while those for Jinjiu Rehmannia glutinosa were 0.024,0.032,and 0.034 g/L,respectively.The study indicates that the total flavonoids from Rehmannia glutinosa possess strong antioxidant activity,which can be used as natural and green antioxidants in animal production.关键词
地黄/总黄酮/菊花心/抗氧化活性Key words
Rehmannia glutinosa/total flavonoids/radial striations/antioxidant activity分类
畜牧业引用本文复制引用
耿晓桐,花娇娇,白向茹,朱乃亮,陈琼..地黄总黄酮提取工艺优化及其抗氧化活性研究[J].饲料研究,2024,47(19):80-85,6.基金项目
河南省创新示范专项(项目编号:201111310900) （项目编号:201111310900）
河南省科技攻关项目(项目编号:232102320294) （项目编号:232102320294）
河南省自然科学基金面上项目(项目编号:232300420065) （项目编号:232300420065）
信阳农林学院青年基金项目(项目编号:QN2022021) （项目编号:QN2022021）
信阳农林学院国家级科研项目培育基金项目(项目编号:pyjj20230101) （项目编号:pyjj20230101）
