饲料研究2024，Vol.47Issue(19)：102-107,6.DOI:10.13557/j.cnki.issn1002-2813.2024.19.020
半边风多酚提取工艺优化及其抗氧化和抗菌活性研究
Study on optimization of extraction process of polyphenols of Plerospermum heterophyllum Hance and its antioxidant and antibacterial activities
摘要
Abstract
To explore the extraction process and antibacterial and antioxidant activities of polyphenols of Plerospermum heterophyllum Hance.This study used microwave-assisted extraction method to optimize the extraction process of polyphenols of Plerospermum heterophyllum Hance based on single factor experiments through response surface methodology.The antioxidant capacity of polyphenols of Plerospermum heterophyllum Hance was measured using hydroxyl radical and DPPH radical scavenging rate as indicators,and the antibacterial capacity was measured using inhibition zone diameter,minimum inhibitory concentration(MIC),and minimum bactericidal concentration(MBC)as indicators.The results showed that the optimal extraction parameters for polyphenols of Plerospermum heterophyllum Hance were ethanol volume fraction of 44％,liquid-solid ratio of 60 mL/g,extraction time of 2.7 minutes,extraction power of 266 W,and polyphenol yield of 1.26％.Compared with L-ascorbic acid,the polyphenols of Plerospermum heterophyllum Hance have a certain hydroxyl radical and DPPH radical scavenging rate,and have antioxidant activity.The diameters of the bacteriostatic circles of polyphenols of Plerospermum heterophyllum Hance against Staphylococcus aureus and Escherichia coli were 9.50 and 8.00 mm respectively,the MIC was 0.469 and 0.938 g/L respectively,and the MBC was 1.875 and 0.938 g/L respectively.The results show that the polyphenols of Plerospermum heterophyllum Hance have both antioxidant and antibacterial activities and can be developed into feed additives for animal production.关键词
半边风/多酚/抗氧化/抗菌Key words
Plerospermum heterophyllum Hance/polyphenols/antioxidant/antibacterial分类
农业科技引用本文复制引用
李泽洋,黄华,肖善芳,郭松..半边风多酚提取工艺优化及其抗氧化和抗菌活性研究[J].饲料研究,2024,47(19):102-107,6.基金项目
广西科技师范学院高层次人才启动经费(项目编号:GXKS2020GKY009) （项目编号:GXKS2020GKY009）
广西高校中青年教师科研基础能力提升项目(项目编号:2021KY0862) （项目编号:2021KY0862）
广西科技师范学院壮瑶药品质生物学重点实验室经费(项目编号:GXKSKYPT2021007) （项目编号:GXKSKYPT2021007）
广西科技基地与人才专项(项目编号:2021AC19423) （项目编号:2021AC19423）
广西高校中青年教师科研基础能力提升项目(项目编号:2023KY0878) （项目编号:2023KY0878）
广西科技师范学院青年科研项目(项目编号:GXKS2022QN102) （项目编号:GXKS2022QN102）
