中国药物经济学2024，Vol.19Issue(10)：109-113,5.DOI:10.12010/j.issn.1673-5846.2024.10.022
纳米胶束靶向递送熊果酸在宫颈癌细胞中的作用及对细胞凋亡的影响
Effect of Targeted Delivery of Ursolic Acid by Nano Micelles on Cervical Cancer Cells and Apoptosis
摘要
Abstract
Objective To investigate the effect of targeted delivery of ursolic acid by nano micelles on cervical cancer cells and its effect on cell apoptosis.Methods Ursolic acid nanomicelles were prepared,observed and characterized.In addition,the drug release rate of ursolic acid nanomicelles in vitro was detected by university chromatography,and the activity of ursolic acid nanomicelles on Hela cells at different doses was evaluated by MTT method,and IC50 was calculated.Hela cells were divided into blank group(normal culture of cervical cancer Hela cells without any intervention),nano micellar empty carrier group(cervical cancer Hela cells and nano micellar empty carrier co-culture),ursolic acid group(cervical cancer Hela cells and 10 µM ursolic acid co-culture)and ursolic acid nanomicellar group(cervical cancer Hela cells and 10 µM sodium ursolic acid co-culture)Rice micelle co-culture).Apoptosis was detected by flow cytometry.The protein levels of apoptosis-related factors Bax,Bcl-2 and Caspase-12 were detected by western blot.Results The characteristics of ursolic acid nanomicelles were observed by transmission electron microscope.The results showed that the micelles were round and spherical,and the size was not uniform.The in vitro drug release curve of ursolic acid nanomicelles from 0.5 to 144 h showed that the release rate of ursolic acid was significantly increased at 24 h,and the drug release rate was 24.60%.After the gradual release stage,the release rate decreased,and the drug release rate was 73.50%at 144 h.Compared with ursolic acid and micellar empty carrier,the cell activity decreased when ursolic acid micellar concentration was 1 μM(P＜0.05).The activity of Hela cells was inhibited from 100%to 15.2%by the concentration of ursolic acid nanomicelles 0～40 µM.The concentration of ursolic acid(0～10 µM)did not significantly inhibit the activity of Hela cells(P＞0.05),but decreased from 20%.There was no significant inhibitory effect on Hela cells with empty micellar carrier(P＞0.05).There were no differences in the apoptosis rate,Bax,Bcl-2 and Caspase-12 protein levels among the blank group,the nanmicelle empty carrier group and the ursolic acid group(P＞0.05).Compared with the ursolic acid group,the apoptosis rate,Bax and Caspase-12 protein levels were increased in the ursolic acid nanmicelle group,while the Bcl-2 protein levels were decreased.There were differences(P＜0.05).Conclusion Compared with ursolic acid alone,nano micellar targeted delivery of ursolic acid can significantly improve the apoptosis of cervical cancer cells,and the mechanism is related to the inhibition of Bcl-2 and the upregulation of Bax/Caspase-12.关键词
宫颈癌/熊果酸/纳米胶束/活性/凋亡Key words
Cervical cancer/Ursolic acid/Nanomicelles/Activity/Apoptosis分类
医药卫生引用本文复制引用
由艳秋,刁志宏,曹步清,谢泷宽,马贞丽,唐芳玫,李福军..纳米胶束靶向递送熊果酸在宫颈癌细胞中的作用及对细胞凋亡的影响[J].中国药物经济学,2024,19(10):109-113,5.基金项目
广西医疗卫生适宜技术开发与推广应用项目(S2022056) （S2022056）
