Mapping and identification of QTLs for seed fatty acids in soybean(Glycine max L.)OACSTPCD
Mapping and identification of QTLs for seed fatty acids in soybean(Glycine max L.)
Soybean is one of the most important sources of vegetable oil.The oil content and fatty acid ratio have attracted significant attention due to their impacts on the shelf-life of soybean oil products and consumer health.In this study,a high-density genetic map derived from Guizao 1 and Brazil 13 was used to analyze the quantitative trait loci of palmitic acid(PA),stearic acid(SA),oleic acid(OA),linoleic acid(LA),linolenic acid(LNA),and oil content(OC).A total of 54 stable QTLs were detected in the genetic map linkage analysis,which shared six bin intervals.Among them,the bin interval on chromosome 13(bin106-bin118 and bin123-bin125)was found to include stable QTLs in multiple environments that were linked to OA,LA,and LNA.Eight differentially expressed genes(DEGs)within these QTL intervals were determined as candidate genes according to the combination of parental resequencing,bioinformatics and RNA sequencing data.All these results are conducive to breeding soybean with the ideal fatty acid ratio for food,and provide the genetic basis for mining genes related to the fatty acid and oil content traits in soybean.
Yiwang Zhong;Hai Nian;Ke Wen;Xingang Li;Shasha Wang;Sansan Li;Yuhong Zeng;Yanbo Cheng;Qibin Ma;Yanyan Wang;Yuanting Pang
State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources,South China Agricultural University,Guangzhou 510642,China||Guangdong Sub-center of the National Center for Soybean Improvement/College of Agriculture,South China Agricultural University,Guangzhou 510642,China||Key Laboratory of Genetic Resources Evaluation and Utilization of Tropical Fmuits and Vegetables,Co-construction by Ministry of Agriculture and Rural Affairs and Hainan Province/Key Laboratory of Vegetable Biology of Hainan Province/Vegetable Research Institute,Hainan Academy of Agricultural Sciences,Haikou 570228,ChinaState Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources,South China Agricultural University,Guangzhou 510642,China||Guangdong Sub-center of the National Center for Soybean Improvement/College of Agriculture,South China Agricultural University,Guangzhou 510642,China||Hainan Seed Industry Laboratory,Sanya 572025,ChinaState Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources,South China Agricultural University,Guangzhou 510642,China||Guangdong Sub-center of the National Center for Soybean Improvement/College of Agriculture,South China Agricultural University,Guangzhou 510642,China||Institute of Genetics and Developmental Biology,Chinese Academy of Sciences,Beijing 100101,China||Hainan Seed Industry Laboratory,Sanya 572025,China||Key Laboratory of Genetic Resources Evaluation and Utilization of Tropical Fmuits and Vegetables,Co-construction by Ministry of Agriculture and Rural Affairs and Hainan Province/Key Laboratory of Vegetable Biology of Hainan Province/Vegetable Research Institute,Hainan Academy of Agricultural Sciences,Haikou 570228,ChinaState Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources,South China Agricultural University,Guangzhou 510642,China||Guangdong Sub-center of the National Center for Soybean Improvement/College of Agriculture,South China Agricultural University,Guangzhou 510642,ChinaInstitute of Genetics and Developmental Biology,Chinese Academy of Sciences,Beijing 100101,ChinaHainan Seed Industry Laboratory,Sanya 572025,China||Beijing Key Laboratory of Growth and Developmental Regulation for Protected Vegetable Crops/Department of Vegetable Science,College of Horticulture,China Agricultural University,Beijing 100193,China
soybeanoilfatty acidsQTLgene
《农业科学学报（英文）》 2024 (012)
3966-3982 / 17
This research was supported by funding from the Seed Industry Revitalization Plan of Guangdong Province,China(2022-NPY-00-007),the Hainan Seed Industry Laboratory,China(B21HJ0901 and B23C1000416),the Key-Area Research and Development Program of Guangdong Province,China(2020B020220008),the National Natural Science Foundation of China(31971966 and 31971965),the China Agricultural Research System(CARS-04-PS09),the National Key Research and Development Projects,China(2018YFE0116900-06),Guangdong Agricultural Science and Technology Innovation and Promotion Project,China(2019KJ136-03),and the Sanya Science and Technology Innovation Special Project,China(2022KJCX11).The authors extend their gratitude to Liying Feng(Yazhou Bay National Laboratory)and Xiaoyu Zhang(South China Normal University)for their valuable assistance with the bioinformatics analysis and English language editing.
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