A novel chorismate mutase effector secreted from root-knot nematode Meloidogyne enterolobii manipulates plant immunity to promote parasitismOACSTPCD
A novel chorismate mutase effector secreted from root-knot nematode Meloidogyne enterolobii manipulates plant immunity to promote parasitism
Meloidogyne spp.is an economically important plant-parasitic nematode distributed worldwide.To fight with host immune system for successful parasitism,plant parasitic nematodes secrete effectors to promote infection.In this study,we identified one chorismate mutase(CM)effector from M.enterolobii,named Me-CM.Spatial and temporal expression assays exhibited Me-cm is expressed in esophageal glands and up-regulated at parasitic-stage juveniles.Me-CM affects the pathogenicity of M.enterolobii based on the reduced infection rate,number of galls,egg masses,eggs per mass and multiplication rate collected from RNA silencing experiments.We showed that Me-CM localized in the cytoplasm and nucleus of plant cells and decreased the expression level of the marker gene PR1 of salicylic acid(SA)pathway.Besides,constitutive expression of Me-cm in Arabidopsis thaliana significantly reduced salicylic acid concentration.These results suggested that M.enterolobii may secrete effector Me-CM to fight with plant immune systems via regulating SA signaling pathway when interacting with host plants,ultimately facilitating parasitism.
Tuizi Feng;Yuan Chen;Zhourong Li;Ji Pei;Deliang Peng;Huan Peng;Haibo Long
Key Laboratory of Integrated Pest Management on Tropical Crops,Ministry of Agriculture and Rural Affairs,Environment and Plant Protection Institute,Chinese Academy of Tropical Agricultural Sciences,Haikou 571101,China||Hainan Key Laboratory for Monitoring and Control of Tropical Agricultural Pests,Environment and Plant Protection Institute,Chinese Academy of Tropical Agricultural Sciences,Haikou 571101,ChinaKey Laboratory of Integrated Pest Management on Tropical Crops,Ministry of Agriculture and Rural Affairs,Environment and Plant Protection Institute,Chinese Academy of Tropical Agricultural Sciences,Haikou 571101,ChinaState Key Laboratory for Biology of Plant Diseases and Insect Pests,Institute of Plant Protection,Chinese Academy of Agricultural Sciences,Beijing 100193,China
Meloidogyne enterolobiieffectorchorismate mutasesalicylic acidplant immunity
《农业科学学报（英文）》 2024 (012)
4107-4119 / 13
This work was supported by the Hainan Provincial Natural Science Foundation of China(323MS102 and 320QN307)and Central Public-Interest Scientific Institution Basal Research Fund,China(1630042022008).We thank to Dr.Jianlong Zhao(Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences)for kindly providing the A.tumefaciens cells carrying Gpa2/RBP1.
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