Vesicular transport-related genes in Diaphorina citri are involved in the process of Candidatus Liberibacter asiaticus infectionOACSTPCD
Vesicular transport-related genes in Diaphorina citri are involved in the process of Candidatus Liberibacter asiaticus infection
Asian citrus psyllid(ACP,Diaphorina citri)is the major vector of Candidatus Liberibacter asiaticus(CLas),which is a bacterial pathogen causing the devastating citrus Huanglongbing(HLB)disease.Diaphorina citri is known to carry CLas in a persistent and propagative manner.Some studies have suggested that CLas may use the vesicular structures of D.citri cells as its propagation organelles.However,the mechanisms by which CLas enters the D.citri cells and how vesicle-mediated trafficking is involved remain unclear.In this study,we monitored the titer change of CLas in D.citri nymphs during the process of CLas acquisition from feeding on infected citrus plants.We found that the titer of CLas increased with the acquisition access period.After infection,there was a significant upregulation in the expression of several vesicular transport-related genes in D.citri.The titer of CLas was significantly reduced in the midgut and whole insect body when endocytosis and the endosome network in D.citri were inhibited.Furthermore,silencing the D.citri clathrin-heavy chain gene also led to a reduction in the CLas titer in D.citri.These results suggest that CLas infection upregulates the genes related to vesicular transport in D.citri,which facilitates the invasion of endocytosis-dependent pathogens.
Yingzhe Yuan;Changyong Zhou;Tao Peng;Aijun Huang;Jun He;Chenyang Yuan;Tianyuan Liu;Long Yi;Xuejin Cui;Xuefeng Wang
National Citrus Engineering and Technology Research Center,Citrus Research Institute,Southwest University,Chongqing 400712,ChinaNational Citrus Engineering and Technology Research Center,Citrus Research Institute,Southwest University,Chongqing 400712,China||College of Life Sciences,Yulin College,Yulin 719000,ChinaNational Navel Orange Engineering Research Center,Gannan Normal University,Ganzhou 341000,ChinaKey Laboratory of Entomology and Pest Control Engineering,College of Plant Protection,Southwest University,Chongqing 400715,China
Huanglongbingendocytosisvesicular traffickingCLas acquisition
《农业科学学报（英文）》 2024 (012)
4136-4146 / 11
This study was supported by grants from the National Key Research and Development Program of China(2021YFD1400800),the National Natural Science Foundation of China(U23A0196 and 32160625),the Innovation Research 2035 Pilot Plan of Southwest University,China(SWU-5331000008),the Special Fund for Youth Team of Southwest University,China(SWU-XJLJ202310)and the Science and Technology Project of Jiangxi Province,China(20225BCJ22005).We thank Dr.Fang Ding(Huazhong Agricultural University,China)for providing the antibody of CLas,and Dr.Binghai Lou(Guangxi Academy of Specialty Crops,China)for providing single D.citri DNA extraction methods.
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