Transcriptome analysis reveals the genetic basis of crest cushion formation in duckOACSTPCD
Transcriptome analysis reveals the genetic basis of crest cushion formation in duck
The Chinese crested duck is a unique duck breed having a bulbous feather shape on its duck head.However,the mechanisms involved in its formation and development are unclear.In the present study,RNA sequencing analysis was performed on the crested tissues of 6 Chinese crested ducks and the scalp tissues of 6 cherry valley ducks(CVs)from 2 developmental stages.This study identified 261 differentially expressed genes(DEGs),122 upregulated and 139 downregulated,in the E28 stage and 361 DEGs,154 upregulated and 207 downregulated in the D42 stage between CC and CV ducks.The subsequent results of weighted gene co-expression network analysis(WGCNA)revealed that the turquoise and cyan modules were associated with the crest trait in the D42 stage,meanwhile,the green,brown,and pink modules were associated with the crest trait in the E28 stage.Venn analysis of the DEGs and WGCNA showed that 145 and 45 genes are associated between the D42 and E28 stages,respectively.The expression of WNT16,BMP2,SLC35F2,SLC6A15,APOBEC2,ABHD6,TNNC2,MYL1,and TNNI2 were verified by real-time quantitative PCR.This study provides an approach to reveal the molecular mechanisms underlying the crested trait development.
Lan Huang;Qixin Guo;Yong Jiang;Zhixiu Wang;Guohong Chen;Guobin Chang;Hao Bai
Joint International Research Laboratory of Agriculture and Agri-Product Safety,Ministry of Education/Institutes of Agricultural Science and Technology Development,Yangzhou University,Yangzhou 225009,China||Key Laboratory of Animal Genetics and Breeding and Molecular Design of Jiangsu Province,College of Animal Science and Technology,Yangzhou University,Yangzhou 225009,ChinaKey Laboratory of Animal Genetics and Breeding and Molecular Design of Jiangsu Province,College of Animal Science and Technology,Yangzhou University,Yangzhou 225009,ChinaJoint International Research Laboratory of Agriculture and Agri-Product Safety,Ministry of Education/Institutes of Agricultural Science and Technology Development,Yangzhou University,Yangzhou 225009,China
crested duckRNA-sequencingweighted gene co-expression network analysisdifferentially expressed genes
《农业科学学报（英文）》 2024 (012)
4172-4185 / 14
This work was supported by the earmarked fund for CARS,China(CARS-42),the earmarked fund for Jiangsu Agricultural Industry Technology System,China(JATS(2022)331)and the Jiangsu Key Research and Development Program,China(BE2021332).
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