烟草赤星病人工接种鉴定新方法研究OA

Tobacco brown spot occurs in the middle and late stages of tobacco growth period and is one of the most threatening leaf diseases in tobacco production.Artificial inoculation is the key to determine the pathogenicity and domi-nant pathogen community of Alternaria alternata,and to identify and screen the disease resistance of tobacco germplasm resources and varieties.In order to explore an efficient method for indoor artificial inoculation of A.alternata,this study provided a new method for identification of artificial inoculation of leaves in vitro,namely spore suspension wound injection method,and compared the inoculation effect of different inoculation methods,as well as the influence of inocu-lation concentration,pathogen age and leaf location on the inoculation of spore suspension wound injection method.The results showed that spore suspension wound injection had the advantages of easy operation,high inoculation rate,short onset period,moderate onset degree and accurate and reliable results.The optimal conditions for inoculation of this new method were A.alternata pathogen age of 10 days,a spore suspension concentration of 1×106 cells/mL,leaves in the lower part of the plant,and an inoculation volume of 10 μL.The new inoculation method can be used as a rapid and accu-rate inoculation method for the identification of pathogenic fungi of tobacco brown spot or other leaf diseases of crops.