茭白WRKY基因家族鉴定及菰黑粉菌侵染下的表达分析OACSTPCD

WRKY gene family is one of the largest transcription factor families in plants and plays an important role in the regulation of plant growth and development and response to stress.In this study,WRKY gene family was identified,and bioinformatic analysis was conducted based on the genome database of Zizania latifolia.Meanwhile,the expression pattern of ZlWRKYs gene after inoculation with Ustilago esculenta was also studied.The results showed that a total of 120 ZlWRKY genes,named ZlWRKY1-ZlWRKY120,were identified in the genome of Z.lati-folia,and they were distributed on chromosome 17.ZlWRKYs could be divided into Groups Ⅰ,Ⅱ and Ⅲ,of which there were 17 ZlWRKYs in Group I,72 ZlWRKYs in Group Ⅱ and 31 ZlWRKYs in Group Ⅲ.Gene fragment duplica-tion existed in ZlWRKYs members.The analysis of promoter cis-acting elements showed that ZlWRKYs contained several elements related to hormones,growth and development and stress response.At 3 h after inoculation with U.esculenta,most ZlWRKYs genes showed up-regulated expression,and the relative expression of ZlWRKY33 was the highest.This results provide a theoretical basis for further studying on the function of ZlWRKYs gene in Z.latifolia and the molecular mechanism of male Z.latifolia response to U.esculenta infection.