安徽农业大学学报2024，Vol.51Issue(5)：796-801,6.DOI:10.13610/j.cnki.1672-352x.20241031.002
TRPM1基因分型及其与乌骨鸡皮肤乌色度关联分析
Genotyping of TRPM1 gene polymorphisms in Silky fowls and its association with skin blackness
摘要
Abstract
This study aimed to genotype the single nucleotide polymorphisms(SNPs)of the TRPM1 gene and analyze the association between these polymorphic sites and skin blackness to identify SNP markers that correlate with skin blackness.The SNPs loci in TRPM1 gene was genotyped using MassARRAY platform based on time-of-flight mass spectrometry analysis technology and the correlation between single SNP markers,and the L*value trait of skin brightness was analyzed.The mass spectrometry detection rate of 6 SNP loci in TRPM1 gene was 100％,and only SNP1 locus(exon 8,rs737038441)had polymorphism,with 3 genotypes.The chi square test showed that the SNP1 locus was in a Hardy Weinberg equilibrium state(P＞0.05),with a PIC of 0.27,indicating moderate polymorphism(0.25＜PIC＜0.5).Single marker association analysis showed that the back skin brightness L*value of the GG genotype at the SNP1 locus was significantly lower than that of the AA and AG genotypes(P＜0.05),while there was no significant difference in the brightness L*value of the thigh and breast skin between dif-ferent genotypes at SNP1 locus.One polymorphic SNP locus(rs737038441 A＞G mutation)of the TRPM1 gene was identified,which was in an H-W equilibrium state.SNP 1 locus of the TRPM1 gene was significantly correlated with the brightness L*value of the back skin of Silky fowls.The GG genotype had a significantly lower brightness L*value of the back skin than that of the AA and AG genotypes(P＜0.05).The GG genotype may be the dominant genotype for the dark color of the back skin of Silky fowls.It will provide a theoretical reference for using molecular marker assisted breeding to accelerate the cultivation of new varieties of high blackness black bone chickens.关键词
乌骨鸡/TRPM1/乌色度/SNP位点/连锁不平衡Key words
black-bone chickens/TRPM1/blackness/SNP sites/linkage disequilibrium分类
农业科技引用本文复制引用
屠云洁,郑国庆,章明,巨晓军,姬改革,刘一帆,单艳菊,邹剑敏,束婧婷,赵伟东..TRPM1基因分型及其与乌骨鸡皮肤乌色度关联分析[J].安徽农业大学学报,2024,51(5):796-801,6.基金项目
江苏省种业振兴揭榜挂帅项目(JBGS[2021]107),现代农业产业技术体系建设专项资金(CARS-41),江苏省农业自主创新基金项目(CX(21)2011-1)和扬州市现代农业项目(YZ2021029)共同资助. （JBGS[2021]107）
