安徽农业大学学报2024，Vol.51Issue(5)：802-807,6.DOI:10.13610/j.cnki.1672-352x.20241031.003
瓦氏黄颡鱼养殖群体的微卫星遗传多样性
Microsatellite genetic diversity in cultured populations of Pelteobagrus vachelli
摘要
Abstract
In order to understand the genetic status of Pelteobagrus vachelli germplasm,which provides a forceful scientific foundation for improved variety breeding of P.vachelli,a total of 168 individuals of P.vachelli were collected from major aquaculture areas(Guangdong,Sichuan,Zhejiang and Anhui Province)in China.The genetic diversity and genetic structure of 6 populations were analyzed using microsatellite markers.The results showed that all populations exhibited high levels of genetic diversity(Na=6.500-8.250,Ho=0.758-0.869,He=0.729-0.806,PIC=0.672-0.762),with similar levels of genetic diversity among them.The genetic distance between populations ranged from 0.057 5 to 0.650 5,the genetic differentiation index from 0.011 9 to 0.152 2,and the proportion of inter-population genetic variation in AMOVA was 9.93％(＞0.05),which indicated that there was significant genetic differentiation among these populations.The phylogenetic tree showed that the 6 populations had a common ancestor type and were clustered into two branches of each other's sister groups,among which 3 populations from Anhui were clustered into one branch and the rest were clustered into another.The results of ge-netic cluster analysis using the software Structure 2.3.4 showed that the optimal number of genetic grouping was 3.The genetic composition of Guangdong,Sichuan and Zhejiang populations was similar,clustering them into one genetic group,which should be the germplasm source of Yangtze River.The genetic composition of Huainan and Lu'an populations was also similar,clustering them into another genetic group,which should be the germplasm source of the main stream of the Huai River.The Fengtai population was a single genetic group,likely representing the germplasm source of the isolated lake,Chengbei Lake,a tributary of the Huai River.There were significant differences in genetic composition among all the genetic groups.The results demonstrated that the six populations of P.vachelli had high genetic diversity and significant genetic differentiation,indicating great poten-tial for selective breeding.The result provides an excellent germplasm bank for the breeding of P.vachelli.关键词
瓦氏黄颡鱼/遗传多样性/微卫星/种质Key words
Pelteobagrus vachelli/genetic diversity/microsatellite/germplasm分类
农业科技引用本文复制引用
胡玉婷,汪焕,段国庆,周华兴,凌俊,江河,潘庭双,杨敏,李彤..瓦氏黄颡鱼养殖群体的微卫星遗传多样性[J].安徽农业大学学报,2024,51(5):802-807,6.基金项目
安徽省水产产业技术体系(皖农科函[2021]711号)和安徽省农业科学院青年英才项目(皖农科人[2022]43号)共同资助. （皖农科函[2021]711号）
