安徽农业大学学报2024，Vol.51Issue(5)：819-825,7.DOI:10.13610/j.cnki.1672-352x.20241031.005
壳寡糖水溶液(优力克)的抗菌活性及其影响因素
Antibacterial activity and influencing factors of chitosan oligosaccharides aqueous solution(Yulike)
摘要
Abstract
To investigate the antibacterial activity in vitro and impact factors of chitosan oligosaccharides aque-ous solution(trade name:Yulike),the standard micro broth dilution combined with plate counting method(referred to as the standard measurement method)was used to determine the minimum inhibitory concentration(MIC)and mini-mum bactericidal concentration(MBC)of Yulike,micro plate simulation whole pool sprinkling(simulated measure-ment method)was used to determine the MIC and MBC of Yulike against MHA cultivable aquatic bacteria,and the effects of incubation temperature,initial bacterial concentration,MH concentration,and pH of the measurement sys-tem,as well as shelf life on Yulike MIC and MBC were evaluated.The results showed that,the MIC and MBC(32-64 g·m-3)of Yulike against Escherichia coli E105,Aeromonas veronii AV040,Bacillus paranthracis BC006,and Ba-cillus velezensis B14 were hundreds of times higher than the clinical recommended concentrations(0.3-0.5 g·m-3);the MIC and MBC values(4-8 g·m-3)of MHA culturable aquatic bacteria by Yulike were dozens of times higher than the clinical recommended concentrations.The higher temperature(5-35 ℃),bacterial concentration(103-108 cfu·mL-1),MH concentration(1/256-1×MH),and pH(5.5-9.5)of the measurement system,the greater the MIC of Yulike against bacteria(the maximum value is 2-8 times the minimum value),and the MIC of Yulike against the four tested bacteria ranged from 16 g·m-3 to 128 g·m-3.The shelf life of Yulike is at least 24 months.These results sug-gested that Yulike is a very promising regulator of bacterial load in the fishing water environment.关键词
壳寡糖/微量稀释联合平板计数法/最小抑菌浓度/最小杀菌浓度Key words
chitosan oligosaccharides/micro dilution combined with plate counting method/minimum in-hibitory concentration/minimum bactericidal concentration分类
农业科技引用本文复制引用
李言,彭开松,熊大华,胡绍愚,刘振红,高梦,羊志军..壳寡糖水溶液(优力克)的抗菌活性及其影响因素[J].安徽农业大学学报,2024,51(5):819-825,7.基金项目
安徽省现代农业产业技术体系项目(皖农科函[2021]711号)和定远县绿色渔业推广与示范2023年度市级科技特派团项目共同资助. （皖农科函[2021]711号）
