安徽农业科学2024，Vol.52Issue(22)：115-117,123,4.DOI:10.3969/j.issn.0517-6611.2024.22.023
尖叶茶藨子种子无菌苗再生体系的建立
Establishment of Rapid Propagation for Sterile Seedlings of Ribes maximowiczianum Komarov by Tissue Culture
摘要
Abstract
The seedlings of mature Ribes maximowiczianum Komarov seed germination under different disinfection treatment were used as ex-plants to study the influence of different plant growth regulators on buds induction,hyperplasia,sound seedling and rhizogenesis.The results showed that 500 mg/L GA3 soaking treatment was conducive to seed germination,and the germination rate could reach 66.7%,and the best disinfection treatment was 75%alcohol for 30s and 0.1%mercuric acid disinfection for 8 min,the contamination rate was 23.3%.The best me-dium for bud induction was MS+2.0 mg/L 6-BA+0.05 mg/L NAA,the proliferation times was 7.5.The best medium for rooting was 1/2MS+2.0 mg/L IBA,the rooting rate was100%,and the average number of rooting shoots was 6.33.The survival rate was 87.5%in the medium of 3∶1∶1 in peat soil∶coir∶perlite.This study established a complete system of tissue culture and rapid propagation for Ribes maximo-wiczianum Komarov seed,which is suitable for the large-scale production and application of Ribes maximowiczianum Komarov seedlings.关键词
尖叶茶藨子种子/组织培养/丛生芽/快速繁育Key words
Seeds of Ribes maximowiczianum Komarov/Tissue culture/Cluster buds/Rapid propagation分类
农业科技引用本文复制引用
林丽霞,林建忠,张雪芹,钟赞华,赖瑞云..尖叶茶藨子种子无菌苗再生体系的建立[J].安徽农业科学,2024,52(22):115-117,123,4.基金项目
厦门市科技计划项目(3502Z20194522) （3502Z20194522）
福建省厦门市重大科技项目(3502Z2019013). （3502Z2019013）
