安徽农业科学2024，Vol.52Issue(22)：162-166,5.DOI:10.3969/j.issn.0517-6611.2024.23.034
Box-Behnken响应面法优选低共熔溶剂提取巴西人参总皂苷工艺
Optimization of Deep Eutectic Solvents Extraction of Total Saponins from Pfaffia glomerata by Box-Behnken Response Surface Meth-odology
摘要
Abstract
[Objective]Response surface methodology was used to optimize the extraction process of total saponins from Pfaffia glomerata,providing reference for its further development and utilization.[Method]Taking amaranthaceous plant Pfaffia glomerata as the research object,total saponins were extracted using ultrasound assisted deep eutectic solvent method.Through single factor experiments,the molar ratio of urea to choline chloride,water content,liquid-solid ratio and extraction time were investigated,the total saponin yield was used as the evaluation in-dex,and the Box-Behnken response surface methodology was used to optimize the extraction process of total saponins from Pfaffia glomerata.[Result]The optimal process for ultrasound-assisted deep eutectic solvents extraction of total saponins from Pfaffia glomerata was established with the following parameters:urea to choline chloride ratio of 1∶1,water content of 38%,liquid-material ratio of 18∶1(mL∶g),and ex-traction time of 37 minutes.Under the conditions,the total saponin yield achieved was 6.26%.[Conclusion]This method has high extraction efficiency,good repeatability and simple operation,and can be used for the extraction of total saponins from Pfaffia glomerata.关键词
巴西人参/低共熔溶剂/总皂苷/提取工艺/响应面法Key words
Pfaffia glomerata/Deep eutectic solvents/Total saponins/Extraction process/Response surface methodology分类
医药卫生引用本文复制引用
董明然,刘佳,夏念桐,李书洋,路娟,陈曦..Box-Behnken响应面法优选低共熔溶剂提取巴西人参总皂苷工艺[J].安徽农业科学,2024,52(22):162-166,5.基金项目
中国医学科学院医学与健康科技创新工程经费(2022-I2M-1-018). （2022-I2M-1-018）
