北京中医药大学学报2024，Vol.47Issue(11)：1481-1489,9.DOI:10.3969/j.issn.1006-2157.2024.11.001
探讨活血养阴法调控Bcl-2/Bax通路改善精索静脉曲张大鼠生精功能的作用机制
Exploring the mechanism of regulating Bcl-2/Bax pathway by the therapy of promoting blood circulation and nourishing yin to improve spermatogenesis in rats with varicocele
摘要
Abstract
Objective To explore the mechanism of the therapy of promoting blood circulation and nourishing yin(PBCNY)in improving spermatogenesis in rats with varicocele based on Bcl-2/Bax pathway.Methods Thirty 8-week-old male SD rats were randomly divided into six groups(five rats per group):sham,model,levocarnitine(1.0 g/kg),and PBCNY low-dose(5.2 g/kg),PBCNY medium-dose(10.4 g/kg),and PBCNY high-dose(20.8 g/kg)groups.Apart from the sham group,all the groups were built a varicocele model by the classical Turner method.After 4 weeks,the sham group and model group were gavaged with normal saline,and the remaining groups were given with levocarnitine or different doses of PBCNY decoction once a day for 4 weeks.At the end of gavage,the sperm quality of rats in each group was detected by a computer-assisted sperm analyzer,and HE staining was used to observe the testicular tissue morphology of rats in each group and performed with Johnsen score.TUNEL method was used to detect apoptosis,RT-qPCR was used to detect Bcl-2 and Bax mRNA expression,and immunohistochemical method was used to detect cytochrome C protein expression.Results Compared to the sham group,sperm total count,motility rate and percentage of sperm with grade(a+b)motility were lower in the model group of rats(P<0.05).HE staining showed disturbed arrangement of seminiferous tubules in the testicular tissue with significant changes and decreased Johnsen score(P<0.05).The result of TUNEL assay showed that the apoptosis rate was increased in the model group(P<0.05).Bax mRNA expression was increased in testicular tissue(P<0.05).The result of immunohistochemistry showed that the positive expression of cytochrome C was increased in the model group(P<0.05).Compared with the model group,the rat sperm total count in the PBCNY medium-dose group,motility rate in the PBCNY low-and medium-dose groups,and percentage of sperm with grade(a+b)motility in the PBCNY low-dose group increased(P<0.05);the pathological structure of rat testis had different degrees of improvement,and Johnsen score increased in the PBCNY medium-dose group(P<0.05);the apoptosis rate of testicular cells decreased in the levocarnitine group and all doses of PBCNY groups(P<0.05);the expression of Bcl-2 mRNA increased in the PBCNY low-dose and high-dose groups(P<0.05),and the expression of Bax mRNA decreased in the levocarnitine group and the PBCNY medium-dose group(P<0.05);the positive expression of cytochrome C decreased in the PBCNY medium-dose group(P<0.05).Conclusion The therapy of PBCNY can improve sperm quality,repair damaged testicular tissue structure and improve spermatogenic function in rats with varicocele,and its mechanism of action may be related to the activation of Bcl-2/Bax pathway and inhibition of cell apoptosis.关键词
活血养阴法/精索静脉曲张/生精功能/细胞凋亡/B淋巴细胞瘤-2/B淋巴细胞瘤-2相关X蛋白通路/大鼠Key words
therapy of promoting blood circulation and nourishing yin/varicocele/spermatogenesis/apoptosis/B-cell lymphoma-2/B-cell lymphoma-2 associated X protein pathway/rats分类
医药卫生引用本文复制引用
梁仪春,王文,梁树麟,付瑞杰,覃湛,耿立果,何超拔,袁少英..探讨活血养阴法调控Bcl-2/Bax通路改善精索静脉曲张大鼠生精功能的作用机制[J].北京中医药大学学报,2024,47(11):1481-1489,9.基金项目
广东省中医药局中医药科研项目面上项目(No.202205111913416600) （No.202205111913416600）
珠海市科技局项目(No.2220004000307,No.2320004000300) Guangdong Provincial Administration of Traditional Chinese Medicine Traditional Chinese Medicine Research Project(No.202205111913416600) （No.2220004000307,No.2320004000300）
