北京中医药大学学报2024，Vol.47Issue(11)：1527-1540,14.DOI:10.3969/j.issn.1006-2157.2024.11.008
化湿润燥方调控Hippo-YAP/TAZ表达对干燥综合征小鼠颌下腺细胞凋亡和功能的影响
Effects of Huashi Runzao Prescription on apoptosis and function of the submandibular gland in Sjögren's syndrome model mice by regulating Hippo-YAP/TAZ expression
摘要
Abstract
Objective To explore the effects of modulating the Hippo-YAP/TAZ pathway using Huashi Runzao Prescription(HRP)on the apoptosis and salivary secretion function of submandibular gland cells in a naive non-obese diabetic(NOD/Ltj)mouse model of Sjögren's syndrome(SS).Methods Eight-week-old female BALB/c mice were selected as the blank group.Eight-week-old female NOD/Ltj were randomly divided into the model,HRP-L,HRP-M,HRP-H,and hydroxychloroquine sulphate(HCQ)groups.After eight weeks of drug administration,the water consumption and salivary flow rate of each group were recorded.The histopathological damage of the submandibular gland in each group was determined using hematoxylin and eosin staining,the apoptosis rate of the submandibular gland was determined using TUNEL staining,and AQP5,Bax,Bcl-2,beclin-1,YAP,p-YAP,and TAZ expressions in submandibular gland tissues were determined using immunohistochemistry and Western blotting.Results Compared with the blank group,the Hippo-YAP/TAZ pathway was inhibited in the submandibular gland tissues of the model group,lymphocyte infiltration increased,water consumption increased,salivary flow rate decreased,AQP5 expression decreased,and submandibular gland cells apoptosis rate increased(P<0.05).Compared with all other administered groups,in the HRP-M group,the Hippo-YAZ/TAZ pathway was significantly activated,lymphocyte infiltration in submandibular gland tissues was reduced,water consumption was reduced,the salivary flow rate and AQP5 expression were increased,and the apoptosis rate of submandibular gland cells was reduced(P<0.05).Conclusion HRP reduced the pathological damage and apoptosis of submandibular gland cells in SS model mice.It improved the overall secretory function of submandibular gland tissues,which may be related to Hippo pathway activation and downregulating YAP/TAZ expression.关键词
化湿润燥方/干燥综合征/细胞凋亡/颌下腺/Hippo信号通路/水通道蛋白5/小鼠Key words
Huashi Runzao Prescription/Sjögren's syndrome/apoptosis/submandibular gland/Hippo signaling pathway/aquaporin 5/mice分类
医药卫生引用本文复制引用
黄子玮,陶庆文,廖佳禾,余新波,贺倩,唐博杰,罗静..化湿润燥方调控Hippo-YAP/TAZ表达对干燥综合征小鼠颌下腺细胞凋亡和功能的影响[J].北京中医药大学学报,2024,47(11):1527-1540,14.基金项目
国家自然科学基金项目(No.81804042) （No.81804042）
首都卫生发展科研专项(No.2024-1-4065) （No.2024-1-4065）
中央高水平医院临床科研业务费专项成果转化项目(No.2023-NHLHCRF-YXHZ-ZRMS-07) （No.2023-NHLHCRF-YXHZ-ZRMS-07）
北京中医药科技发展基金项目(No.BJZYQN-2023-04) （No.BJZYQN-2023-04）
中日友好医院"菁英计划"人才培育工程(No.ZRJY2024-GG08) （No.ZRJY2024-GG08）
中华中医药学会风湿病分会青年培英计划项目(No.202327-007) National Natural Science Foundation of China(No.81804042) （No.202327-007）
