草业学报2024，Vol.33Issue(11)：69-83,15.DOI:10.11686/cyxb2023475
虎尾草伴生和丛枝菌根真菌对碱胁迫下羊草生长的影响
Effects of Chloris virgata and arbuscular mycorrhizal fungi on the growth of Leymus chinensis under alkali stress
摘要
Abstract
Leymus chinensis and Chloris virgata are common plants that are able to tolerate the barren,saline-alkali soils of the Songnen grassland.This research investigated the effects of C.virgata and arbuscular mycorrhizal(AM)fungi on the alkali tolerance of L.chinensis.A pot experiment was conducted with L.chinensis as the host plant,C.virgata as the companion species,and AM fungal communities derived from the rhizosphere of L.chinensis from Songnen saline-alkali grassland as the inoculant.The experiment therefore had three variables:AM fungi,a companion plant,and alkali stress.The mycorrhizal infection rate,growth index,leaf chlorophyll content,malondialdehyde(MDA)content,antioxidant enzyme activity,and osmoregulatory substance content of L.chinensis were measured to analyze the effects of the companion plant and AM fungal inoculation on the physiology and growth of L.chinensis under alkali stress.The main results were as follows:1)The AM fungi effectively colonized the roots of L.chinensis under alkali stress,and C.virgata inhibited this symbiosis.2)Compared with uninoculated L.chinensis,plants of L.chinensis inoculated with AM fungi showed a significantly reduced MDA content,and increased biomass,chlorophyll content,antioxidant enzyme activities,and osmoregulatory substance contents,which resulted in enhanced alkali tolerance.3)The biomass of L.chinensis was lower in the control than in the treatment groups.Compared with L.chinensis in the control group,L.chinensis in the alkali treatment group showed higher contents of osmoregulatory substances,increased enzyme activities,and improved growth.4)The interaction between C.virgata and AM fungi was associated with increased leaf width,saturation fresh weight,chlorophyll content,soluble sugar content,and soluble protein content,and enhanced activities of peroxidase and catalase in L.chinensis.5)A membership function analysis showed that AM fungi can interact with C.virgata and improve the tolerance of L.chinensis in alkali habits.These results provide a theoretical basis for evaluating the effects of AM fungi and C.virgata as a companion plant on the growth of L.chinensis under alkali stress.These findings also provide important data to assist development of methodologies for the ecological restoration of Songnen saline-alkali grassland by introduction of appropriate companion plants and exploitation of mycorrhizal symbioses.关键词
羊草/虎尾草/丛枝菌根真菌/碱胁迫Key words
Leymus chinensis/Chloris virgata/arbuscular mycorrhizal fungi/alkali stress引用本文复制引用
卢晓瑜,刘雅洁,白彩霞,李进华,王子贺,杨春雪..虎尾草伴生和丛枝菌根真菌对碱胁迫下羊草生长的影响[J].草业学报,2024,33(11):69-83,15.基金项目
国家自然科学基金青年科学基金项目(31601986)和黑龙江省博士后科研启动基金项目(LBH-Q16005)资助. （31601986）
