草业学报2024，Vol.33Issue(11)：106-122,17.DOI:10.11686/cyxb2023490
紫花苜蓿BZR基因家族鉴定及其对非生物胁迫的响应分析
Identification of the BZR gene family in alfalfa and analysis of its transcriptional responses to abiotic stress
摘要
Abstract
Brassinosteroids(BRs)are a class of steroid hormones that influence various developmental and physiological processes in plants.Members of the BRASSINAZOLE-RESISTANT(BZR)transcription factor family are positive regulators of brassinosteroid biosynthesis.The BZR gene family plays a crucial role in regulating plant growth and development,including various aspects of stress resistance.However,the BZR gene family in alfalfa(Medicago sativa),which is known as the'king of forages',has not been thoroughly studied.In this study,we identified 14 BZR genes in alfalfa,and used bioinformatics tools to analyze the physicochemical properties and secondary and tertiary structures of their encoded proteins.The chromosomal locations,phylogenetic relationships,structures,conserved domains,promoter cis-acting elements,gene family interactions,and transcript levels of the 14 genes in six different tissues of alfalfa,as well as their transcriptional responses to abiotic stresses,were also determined.We found that all 14 BZR genes were expressed in various tissues of alfalfa,with nine genes responding to cold stress,seven responding to salt stress,and seven genes responding drought stress.Notably,three BZR genes(MsBZR05,MsBZR06,and MsBZR12)responded to all three types of stress.The results of this study highlight candidate genes for further research on the BZR gene family.关键词
BZR基因家族/紫花苜蓿/生物信息学分析/非生物胁迫/表达分析Key words
BZR gene family/Medicago sativa/bioinformatics analysis/abiotic stress/expression analysis引用本文复制引用
黎泽斌,邱永争,刘延杰,喻金秋,王柏吉,刘千宁,王月,崔国文..紫花苜蓿BZR基因家族鉴定及其对非生物胁迫的响应分析[J].草业学报,2024,33(11):106-122,17.基金项目
国家重点研发计划专项(2022ZD040120401)和国家自然科学基金项目(32301487)资助. （2022ZD040120401）
