南方医科大学学报2024，Vol.44Issue(11)：2082-2091,10.DOI:10.12122/j.issn.1673-4254.2024.11.04
慢性不可预知温和应激诱导的抑郁小鼠中央杏仁核和边缘前皮质神经网络的可塑性变化
Neuronal plasticity changes in the central amygdala and prelimbic cortex network in mice with chronic unpredictable mild stress-induced depression
摘要
Abstract
Objective To explore the relationship between alterations of neural network plasticity and spatial learning and memory functions in mouse models with depression-like behaviors.Methods C57Thy1-YFP/GAD67-GFP mice were randomized into control group(with no treatment)and chronic unpredictable mild stress(CUMS)group(n=15)subjected to CUMS for 8 weeks.Depression-like behaviors of the mice were assessed using sucrose preference test,open field test,and forced swimming test,and their spatial learning and memory abilities were evaluated using Morris water maze test.The changes in the firing patterns of different neuronal subtypes were detected in the central nucleus of the amygdala(CeA)and the prelimbic cortex(PrL)using whole-cell patch-clamp technique.Results Compared with the control mice,CUMS mice showed significantly decreased sucrose preference,total distance moved,number of grid-crossings,entries into the central area,and time spent in the central area in the open field test(P<0.01).In the forced swimming test,CUMS mice exhibited obviously shortened time of struggling,swimming,and climbing with increased immobility time.In Morris water maze test,CUMS mice showed significantly increased escape latency and path length,decreased percentage of distance and swimming time within the target quadrant,and increased first entry latency into the target zone and swimming time within the opposite quadrant.Exposure to CUMS resulted in significantly enhanced energy barrier and increased absolute refractory period and inter-spike interval of glutamatergic neurons in the CeA and GABAergic neurons in the PrL,while the opposite changes were observed in GABAergic neurons in the CeA and glutamatergic neurons in the PrL.Conclusion CUMS-induced depression may lead to plastic changes in the excitatory and inhibitory neuronal networks within the CeA and PrL to cause impairment of spatial learning and memory abilities in mice.关键词
抑郁/可塑性/杏仁核/内侧前额叶皮层Key words
depression/neuroplasticity/amygdala/medial prefrontal cortex引用本文复制引用
刘冬播,陈泽文,王云,李昕鹏,赵彭玉,郑浩贤..慢性不可预知温和应激诱导的抑郁小鼠中央杏仁核和边缘前皮质神经网络的可塑性变化[J].南方医科大学学报,2024,44(11):2082-2091,10.基金项目
安徽省高校自然科学研究重点项目(2023AH051937) （2023AH051937）
