南方医科大学学报2024，Vol.44Issue(11)：2102-2109,8.DOI:10.12122/j.issn.1673-4254.2024.11.06
电针预处理通过抑制NF-kB/NLRP3信号通路介导炎症和凋亡改善大鼠脑卒中后痉挛
Electroacupuncture pretreatment alleviates post-stroke spasticity in rats by inhibiting NF-κB/NLRP3 signaling pathway-mediated inflammation and neuronal apoptosis
摘要
Abstract
Objective To explore the mechanism of electroacupuncture pretreatment(EP)for relieving post-stroke spasticity in rats.Methods Eighteen rats were randomized equally into sham-operated group,middle cerebral artery occlusion(MCAO)group,and MCAO+EP group.In MCAO+EP group,the rats received electroacupuncture at the acupoints Qubin and Baihui for 3 consecutive days prior to MCAO.Neurological deficits and cognitive function of the rats were evaluated,and pathologies in the hippocampus were examined using HE,Nissl,and TUNEL staining.The expressions of IL-4,IL-6,TNF-α,and TMAO in the brain tissues were detected with ELISA,and the mRNA and protein expression levels of NF-κB p65,NLRP3,caspase-3,and caspase-9 were determined with qRT-PCR,Western blotting,and immunohistochemistry.Results The rats receiving MCAO had significantly increased neurological deficit scores and showed increased muscle tension,number of apoptotic neurons,and expressions of IL-6,TNF-α,NF-κB p65,NLRP3,caspase-3 and caspase-9 in the hippocampus and significantly reduced length of time for new object recognition.Microscopically,the cells in the hippocampus of the MCAO rats showed uneven and loosened arrangement and unclear cell boundaries.In contrast,the rats in I/R+EP group showed significantly lowered neurological deficit scores and dystonia rating scores,reduced cell apoptosis,lowered hippocampal expressions of IL-6,TNF-α,caspase-3,caspase-9,and NF-κB p65,increased time for new object recognition,tightly arranged and uniformly stained hippocampal cells with clear boundaries,with also an increased number of active neurons and enhanced expression of IL-4 in the hippocampus.Conclusion EP alleviates post-stroke spasticity in rats by inhibiting inflammatory responses and hippocampal neuronal apoptosis mediated by the NF-κB/NLRP3 signaling pathway.关键词
脑卒中后痉挛/电针预处理/NF-κB/NLRP3信号通路/炎症反应/细胞凋亡Key words
spasticity after stroke/electroacupuncture/NF-κB/NLRP3/inflammatory response/apoptosis引用本文复制引用
孙秀颀,蔡静,张安邦,庞博,陈春艳,查琪琪,全菲,叶涛..电针预处理通过抑制NF-kB/NLRP3信号通路介导炎症和凋亡改善大鼠脑卒中后痉挛[J].南方医科大学学报,2024,44(11):2102-2109,8.基金项目
国家自然科学基金(82060896) （82060896）
贵州省科技计划基础研究(科学技术基金)项目(黔科合基础-ZK[2021]一般505) （科学技术基金）
贵州省教育厅青年科技人才成长项目(黔教合KY字[2021]210) （黔教合KY字[2021]210）
贵州省中医药管理局中医药、民族医药科学技术研究课题(QZYY-2021-015) （QZYY-2021-015）
贵州省卫生健康委科学技术基金项目(gzwkj2021-072)Supported by National Natural Science Foundation of China(82060896). （gzwkj2021-072）
