南方医科大学学报2024，Vol.44Issue(11)：2121-2130,10.DOI:10.12122/j.issn.1673-4254.2024.11.08
五谷虫通过抑制免疫应激-补体活化缓解咪喹莫特诱导的小鼠银屑病样皮肤损伤
Maggot alleviates imiquimod-induced psoriasis-like skin lesions in mice by inhibiting immune stress and complement activation
摘要
Abstract
Objective To explore the therapeutic mechanism of maggot for psoriasis-like lesions in mice from the perspective of immune stress and complement activation regulation.Methods Thirty-six male C57BL/6 mice were randomly divided into control group,model group,maggot(1.25%,2.5%,and 5%)groups,and Benvitimod(1%)group.Psoriasis-like lesions were induced by application of imiquimod cream,and the severity of skin lesions was assessed using the modified Psoriasis Area and Severity Index(MPASI)score.Auricular swelling of the mice was observed,and histopathological changes of the skin lesions were examined with HE staining.Scratching behavior of the mice was observed and the spleen index was calculated.Toluidine blue staining was used to detect mast cells in the skin lesions,and serum levels of IgG,IgM,the complements CH50,C1s,C3,C3a,C5 and C5a,and the inflammatory factors IL-23,IL-17A and TNF-α were determined with ELISA.Results In mice with imiquimod-induced psoriasis-like skin lesions,treatment with the maggot at the 3 doses significantly decreased MPASI score,alleviated auricular swelling and pathologies in the skin lesions,reduced scratching behaviors,spleen index,and the number of mast cells in the lesions.Treatment with high-dose maggot significantly lowered serum levels of IgG,C1s,C3a,C5a,IL-23,IL-17A and TNF-α and the levels of C1s,C3,C3a,C5 and C5a in the lesion tissue,and increased serum levels of CH50,C3,and C5.The therapeutic effect of maggot showed a dose-effect dependence.Conclusion Maggot can alleviate psoriasis-like skin lesions in mice by inhibiting immune stress and complement activation.关键词
五谷虫/银屑病/免疫应激/补体活化/炎症反应Key words
maggot/psoriasis/immune stress/complement activation/inflammatory response引用本文复制引用
姚宏,卫培峰,段佳林,奚苗苗,刘珂娣,刘澄曌,李伟红,代旗,赵石,丁子恒,王鹤霏,葛晓静..五谷虫通过抑制免疫应激-补体活化缓解咪喹莫特诱导的小鼠银屑病样皮肤损伤[J].南方医科大学学报,2024,44(11):2121-2130,10.基金项目
上海市2022年度"科技创新行动计划"生物医药科技支撑专项(22S21902600) （22S21902600）
