福建农业科技2024，Vol.55Issue(9)：23-29,7.DOI:10.13651/j.cnki.fjnykj.2024.09.005
大肠杆菌过表达WcaB对氨基糖苷类抗生素敏感性的影响
Effect on the Sensitivity of Escherichia coli Overexpressing WcaB to Aminoglycoside Antibiotics
摘要
Abstract
In order to find the new targets to accelerate the solution of bacterial resistance problem caused by the antibiotic abuse,Escherichia coli BW25113 overexpressing acetyltransferase WcaB strain was constructed by using the pCA24N expression vector,and the killing effect of aminoglycoside antibiotics was tested in the logarithmic phase.Then,the minimum inhibitory concentration(MIC)and growth curve of Escherichia coli BW25113 overexpressing WcaB to aminoglycoside antibiotics were further determined.The results showed that:the overexpression of WcaB would inhibit the bacterial growth,and the MIC of aminoglycoside antibiotics to WcaB overexpressing strain did not change significantly.The overexpression of WcaB reduced the survival rate of bacteria treated with gentamicin or kanamycin,indicating that the overexpression of WcaB increased the sensitivity of Escherichia coli to aminoglycoside antibiotics.The results showed that acetyltransferase played an important role in enhancing the sensitivity of antibiotics to Escherichia coli,and the enzyme was expected to become a new pharmacological target for Escherichia coli.关键词
乙酰基转移酶/氨基糖苷类抗生素/大肠杆菌Key words
Acetyltransferase/Aminoglycoside antibiotics/Escherichia coli分类
农业科技引用本文复制引用
俞丹丹,张裕丹,翁丽红,高媛媛..大肠杆菌过表达WcaB对氨基糖苷类抗生素敏感性的影响[J].福建农业科技,2024,55(9):23-29,7.基金项目
福建省自然科学基金面上项目(2024J01295). （2024J01295）
