福建农业科技2024，Vol.55Issue(9)：36-43,8.DOI:10.13651/j.cnki.fjnykj.2024.09.007
D-海藻糖增强氨基糖苷类抗生素杀灭大肠杆菌的机制探究
Study on the Mechanism of D-trehalose Enhancing the Killing of Escherichia coli by Aminoglycoside Antibiotics
摘要
Abstract
Finding the efficient antibiotic adjuvants and studying their synergistic bactericidal mechanism is one of the effective strategies to deal with the problem of bacterial resistance.In order to explore the mechanism of D-trehalose enhancing the killing effect of Escherichia coli by the aminoglycoside antibiotics,by taking Escherichia coli BW25113 as the research object,the contents of ATP,NADH and proton motive force(PMF)were measured before and after the treatment of D-trehalose combined with the aminoglycoside antibiotics.The changes of energy metabolism and membrane potential in bacteria were analyzed,and the effect on the uptake of antibiotics was detected by using the inhibition zone experiment.The results showed that:after the double treatment with D-trehalose combined with tobramycin or gentamycin,D-trehalose could increase the synthesis of ATP,increase the level of NADH,and promote the uptake of antibiotics by increasing the PMF level of Escherichia coli,thus significantly enhancing the bactericidal effect of antibiotics.The results of this study provided a basis for the development of D-trehalose as a new-type aminoglycoside antibiotic adjuvant.关键词
大肠杆菌/D-海藻糖/妥布霉素/庆大霉素/质子动力势Key words
Escherichia coli/D-trehalose/Tobramycin/Gentamycin/Proton motive force分类
农业科技引用本文复制引用
黄炜雅,钟洁亚,赖泽迎,陈慧君,赵航宇,陈雅娟..D-海藻糖增强氨基糖苷类抗生素杀灭大肠杆菌的机制探究[J].福建农业科技,2024,55(9):36-43,8.基金项目
福建省自然科学基金面上项目(2024J01457). （2024J01457）
