寒旱农业科学2024，Vol.3Issue(11)：1055-1059,5.DOI:10.3969/j.issn.2097-2172.2024.11.014
纤维素降解菌的分离筛选及其秸秆降解能力研究
Isolation and Screening of Cellulose Degrading Bacteria and Their Straw Degradation Capacity
摘要
Abstract
In order to screen for efficient cellulose degrading bacteria for straw,so as to provide high-quality microbial resources for the further resource utilization of crop straw,dairy cow dung samples were collected,and the strains were initially screened by cellulose plate Congo red staining and rescreened by cellulase and xylanase activity measurement in fermentation broth to explore the degradation capacity of the selected strains on straw.Results showed that 9 strains of cellulase-producing bacteria were obtained,among which strain TX1 exhibited the largest hyaline circle to colony diameter ratio of 1.729.Strain TX7 had the strongest cellulase and xylanase activity,with a CMC-Na enzyme(CX)activity of 0.224 7 U/mL,a filter paper enzyme(FPA)activity of 0.296 0 U/mL,and a xylanase activity of 8.84 U/mL.Strain TX3 showed the strongest degradation capacity for hemicellulose and cellulose in wheat straw,with cellulose and hemicellulose masses of 0.185 g and 0.190 g,respectively,per 1.000 g of wheat straw.Strain TX7 exhibited the strongest degradation capacity for lignin in wheat straw,with a lignin mass of only 0.037 g per 1.000 g of wheat straw.Thus,strains TX3 and TX7 have the potential for further development into efficient strawdegrading microbial agents,providing high-quality microbial resources for the conversion and utilization of straw cellulose.关键词
纤维素降解菌/秸秆/筛选/降解能力/奶牛粪Key words
Cellulose degrading bacteria/Straw/Screening/Degradation capacity/Dairy cow dung分类
资源环境引用本文复制引用
汤莹,赵旭,李娟,王子宁..纤维素降解菌的分离筛选及其秸秆降解能力研究[J].寒旱农业科学,2024,3(11):1055-1059,5.基金项目
中央引导地方科技发展资金项目(23YQA0323) （23YQA0323）
甘肃省现代农业科技支撑体系区域创新中心重点科技项目(2023GAAS07) （2023GAAS07）
甘肃省农业科学院科技成果转化项目(2024GAAS-CGZH04). （2024GAAS-CGZH04）
