南方农业学报2024，Vol.55Issue(9)：2632-2640,9.DOI:10.3969/j.issn.2095-1191.2024.09.009
光合细菌沼泽红假单胞菌Atp2蛋白对水稻生长及抗逆的影响
Effects of photosynthetic bacteria Rhodopseudomonas palustris Atp2 protein on growth and stress resistance in rice
摘要
Abstract
[Objective]To investigate the effects of the photosynthetic bacteria Rhodopseudomonas palustris PSB06 Atp2 protein on the growth,defence enzyme activity and the transcript levels of defense-related genes in rice,and to pro-vide theoretical basis for the popularization of the use of biocontrol bacteria in rice.[Method]Rice variety CO-39 was used as the test material,and rice seeds and leaves were treated with 3 treatment solutions of 0(distilled water,CK),20 and 50 μg/mL Atp2 protein,the chlorophyll a/b and the hydrogen peroxide(H2O2)content were detected,as well as the activities of rice defense-related enzymes such as peroxidase(POD),catalase(CAT),superoxide dismutase(SOD),glutathione reductase(GR),phenylalanine ammonia-lyase(PAL),polyphenol oxidase(PPO)in rice,and the transcript levels of defense-related genes(OsWRKY70,OsLOX1,OsAOS2,OsPR1a,OsCHT1 and OsPAL1)in rice were detected by real-time fluorescence quantitative PCR.[Result]Compared with CK,chlorophyll a/b was significantly(P<0.05,the same below)or extremely significantly(P<0.01,the same below)increased in rice after Atp2 protein treatment of rice leaves,while chlorophyll a/b did not change significantly after treatment of seeds(P>0.05);H2O2 content in rice was ele-vated in all Atp2 protein treatments,with extremely significant elevation in the content of H2O2 after treatment of leaves;POD and CAT activities in rice were significantly or extremely significantly decreased after Atp2 protein treatment;SOD,GR and PAL activities in rice were extremely significantly increased after Atp2 protein treatment;PPO activity in rice was significantly or extremely significantly increased after Atp2 protein treatment of seeds,whereas PPO activity was signifi-cantly or extremely significantly decreased after treatment of leaves.After Atp2 protein treatment,expressions of rice de-fense genes OsWRKY70,OsLOX1,OsAOS2(except 50 μg/mL Atp2 protein treated rice leaves),OsPR1a and OsCHT1 were all up-regulated,of which the expression of OsWRKY70,OsLOX1 and OsCHT1 genes were either significantly or extremely significantly up-regulated;the expression of OsPAL1 gene was extremely significantly up-regulated after the treatment of seeds with Atp2 protein,and OsPAL1 gene expression was extremely significantly up-regulated after the treat-ment of leaves.[Conclusion]The R.palustris PSB06 Atp2 protein of R.palustris has good growth-promoting and stress-resistant effect on rice,which has the potential for promotion and application.关键词
光合细菌/沼泽红假单胞菌/水稻/Atp2蛋白/防御酶活/防御相关基因Key words
photosynthetic bacteria/Rhodopseudomonas palustris/rice/Atp2 protein/defensive enzyme activity/defense-related genes分类
农业科技引用本文复制引用
陈春燕,黄强,吴希阳,覃莹菲,谭新球,刘勇,陈岳,张德咏..光合细菌沼泽红假单胞菌Atp2蛋白对水稻生长及抗逆的影响[J].南方农业学报,2024,55(9):2632-2640,9.基金项目
国家自然科学基金项目(31972323) （31972323）
湖南省科技创新计划项目(2021RC3114) （2021RC3114）
湖南省农业科技创新资金项目(2023CX04) National Natural Science Foundation of China(31972323) （2023CX04）
Hunan Science and Technology Inno-vation Project(2021RC3114) （2021RC3114）
Hunan Agricultural Science and Technology Innovation Project(2023CX04) （2023CX04）
