南方农业学报2024，Vol.55Issue(9)：2744-2753,10.DOI:10.3969/j.issn.2095-1191.2024.09.019
基于品质指标的正交试验优化铁皮石斛真空冷冻干燥工艺
Orthogonal experimental optimization of Dendrobium officinale Kimura et Migo vacuum freeze-drying process based on quality indexes
摘要
Abstract
[Objective]Optimized the time parameters based on the existing industrial production process of vacuum freeze-drying for Dendrobium officinale Kimura et Migo,aiming to obtain the best drying technology,enhance efficiency and product quality,serving as a reference for the industrial production of dried D.officinale products.[Method]Using fresh D.officinale as raw material,an orthogonal experimental design was employed to investigate the effects of sublima-tion durations on the contents of D.officinale polysaccharides,mannose,alcohol extract,polyphenols,as well as antioxi-dant activity.The results were compared with those obtained from the current industrial vacuum freeze-drying process to determine the optimal conditions for vacuum freeze-drying.[Result]The order of the main factors affecting the quality of vacuum freeze-dried D.officinale was as follows:time used at 35℃>time used at 55℃>time used at 25℃>time used at 45℃.The optimal vacuum freeze-drying parameters were a vacuum degree of 30 Pa,cold trap temperature of-40℃,sublimation temperatures of 15℃(3 h)→ 25℃(3 h)→ 35℃(5 h)→ 45℃(9 h)→ 55℃(18 h),totaling 38 h.Un-der these conditions,D.officinale polysaccharide content was 35.3%,mannose content was 23.1%,alcohol extract con-tent was 7.8%,1,1-diphenyl-2-picryl-hydrazyl radical(DPPH)free radical scavenging rate was 30.3%,and a comprehen-sive score of 88.3.Compared to the existing process,drying time was reduced by 2.6%,polysaccharide content and man-nose content were significantly increased by 27.0%and 5.0%respectively(P<0.05,the same below),alcohol extract con-tent had no significant difference(P>0.05),DPPH free radical scavenging rate was significantly decreased by 31.0%,with equivalent polyphenol content and a color closer to that of fresh D.officinale samples.[Conclusion]The optimized vacuum freeze-drying process resulted in D.officinale with higher active ingredient content,reduced drying time,and im-proved color quality.关键词
铁皮石斛/真空冷冻干燥/品质/正交试验/最优组合Key words
Dendrobium officinale Kimura et Migo/vacuum freeze-drying/quality/orthogonal experiment/opti-mal combination分类
农业科技引用本文复制引用
方良材,廖圆圆,孙奇,刘梦姣,吴钊龙,陈俊,黄浩,吴秋萍,覃建维,苏静..基于品质指标的正交试验优化铁皮石斛真空冷冻干燥工艺[J].南方农业学报,2024,55(9):2744-2753,10.基金项目
广西创新驱动发展专项(桂科AA18118015-2) （桂科AA18118015-2）
广西高校中青年教师科研基础能力提升项目(2020KY36009) Guangxi Innovation Driven Development Special Project(Guike AA18118015-2) （2020KY36009）
Middle-aged and Young Teachers'Basic Ability Prmotion Project of Guangxi Universities(2020KY36009) （2020KY36009）
