南方农业学报2024，Vol.55Issue(9)：2813-2823,11.DOI:10.3969/j.issn.2095-1191.2024.09.025
急性低温和复温对青田田鱼鳃组织代谢的影响
Effects of acute cold stress and rewarming on the metabolism of gill tissue of Qingtian paddy field carp(Cyprinus carpio var.qingtianensis)
摘要
Abstract
[Objective]Qingtian paddy field carp(Cyprinus carpio var.Qingtianensis,PF carp)was frequently ex-posed to the stress of acute cold stress in ambient temperatures in shallow water environments.The study aimed to investi-gate the physiological response mechanism of PF carp in acute cold stress and rewarming,which could provide basis for scientific overwintering of PF carp and breeding of low-temperature-tolerant varieties.[Method]The metabolic peaks in gill tissue of PF carp were extracted by liquid chromatography-mass spectrometry(LC-MS)non-targeted metabolomics in the 9.0℃ cold stress for 6 h(CO group)and subsequent rewarming recovery to 28.0℃ for 6 h(RE group)and the 28.0℃ control group(Con group).The pre-processed metabolomics data were matched with the database to identify the metabolites.The reliability of metabolomics data was identified by principal component analysis(PCA)and orthogonal least-squares discriminant analysis(OPLS-DA).The differential metabolites between groups were screened according to the P value of Student's test(P<0.05)and the variable weight value of OPLS-DA model(VIP>1).The KEGG signal pathway enrichment analysis of differential metabolites was performed.The metabolic pathways mostly associated with acute cold stress and rewarming were screened by Fisher test.Cluster heat maps were made for important metabolic path-ways to show the differences between groups and the changing trend of metabolite content.[Result]The PCA and OPLS-DA analysis showed that samples were aggregated within group,and were separated between groups.There were signifi-cant differences in metabolite levels between groups.The OPLS-DA score chart showed that the cumulative Q2 values of all groups were above 0.5,and the cumulative R2Y values were close to 1.0.The model had high interpretability and reliability.A total of 1222 metabolites were identified,among which 232,238,300 significantly different metabolites were screened and identified respectively in CO vs Con,RE vs CO and RE vs Con(P<0.05).Differential metabolism between groups were mainly enriched in metabolic pathways such as arachidonic acid metabolism,glycerophospholipid metabolism,steroid hormone biosynthesis and purine metabolism.Specifically,under acute cold stress,cortisol levels was up-regulated,but arachidonic acid and its metabolites,glycerophospholipid metabolites were down-regulated.After rewarming,the metabolism of arachidonic acid and glycerophospholipid,purine metabolism were down-regulated com-pared with Con group,while the content of cortisol was down-regulated and the content of taurine was up-regulated.[Conclusion]Acute cold stress can lead to immunosuppression,abnormalities in substance transport and signal transmis-sion in PF carp.Cortisol synthesis and metabolism are important mechanisms for resistance to acute cold stress.Gill tissue damage can not be fully recovered in the short term after rewarming.Taurine synthesis may be an important recovery mechanism of PF carp after rewarming.关键词
青田田鱼/急性低温/复温恢复/代谢组学/鳃组织Key words
Qingtian paddy field carp(Cyprinus carpio var.qingtianensis)/acute cold stress/rewarming recovery/metabolomics/gill tissue分类
农业科技引用本文复制引用
廖嘉仪,熊梓彤,李志力,刘其根,孙佳敏..急性低温和复温对青田田鱼鳃组织代谢的影响[J].南方农业学报,2024,55(9):2813-2823,11.基金项目
国家自然科学基金项目(32172995) （32172995）
上海市科学技术委员会能力建设项目(22010502100) （22010502100）
上海市浦江人才项目(22PJ1404500) National Natural Science Foundation of China(32172995) （22PJ1404500）
Ability Building Project of Shanghai Committee of Science and Technology(22010502100) （22010502100）
Shanghai Pujiang Talent Project(22PJ1404500) （22PJ1404500）
