基于网络药理学分析槲皮素治疗细菌性肠炎的潜在机制OA

To explore the potential mechanism of quercetin in the treatment of bacterial enteritis based on network pharmacology and molecular docking techniques,the target of quercetin was obtained by searching TCMSP and SwissTarget databases.The GeneCard database was used to screen the related targets of bacterial enteritis.By comparing the targets of quercetin and bacterial enteritis,the potential targets of quercetin in the treatment of bacterial enteritis were obtained.STRING database was used for protein interaction network analysis,and Cytoscape3.7.2 software was used to construct related protein interaction network,and key nodes were screened for visual analysis.GO function enrichment and KEGG signal path enrichment analysis were conducted using Metascape database.Finally,the molecular docking between quercetin and target gene was verified by AutoDock software.The results showed that 216 quercetin targets and 4 103 bacterial enteritis targets were obtained,with a total of 156 targets.The PPI network involved 149 nodes and 1 078 interaction lines,and the visualization results showed that the protein interaction frequency was higher in TP53,AKT1,JUN,HSP90AA1,SRC,etc.A total of 2 157 items with significant differences were obtained by GO functional enrichment analysis(P＜0.01),and 201 items with significant differences were obtained by KEGG pathway enrichment analysis(P＜0.01),mainly involving PI3K-Akt signaling pathway,AGE-RAGE signaling pathway,TNF signaling pathway,IL-17 signaling pathway,etc.Molecular docking results showed that quercetin had good binding activity with AKT1,IL-6,MAPK1 and RELA.By means of network pharmacology and molecular docking,the potential target of quercetin in the treatment of bacterial enteritis was successfully identified,and the key pathway of its pharmacological action was predicted.