过表达AtVIP1基因增强转基因拟南芥对铜胁迫的抗性OA

Copper is crucial for plant growth,and excessive amounts can lead to a decrease in biomass.AtVIP1 belongs to the bZIP family of Arabidopsis thaliana,which has the functions of starch accumulation,osmotic signaling and promoting Agrobacterium transformation.The transcriptional factor VIP1 has been reported in plant response to abiotic stress,but the study related to copper stress has not been reported.To clarify the role of overexpression of AtVIP1 gene in the response of plants to copper stress,50 μM copper ion was added to 1/2 MS solid medium to observe the effects of overexpression of AtVIP1 gene on seed germination and seedling growth after stress.The results showed that under copper stress,overexpression of AtVIP1 gene could alleviate the inhibition of seed germination,alleviate the damage to seedling growth,increase the activity of resistant enzymes such as SOD,POD and CAT,reduce MDA content,reduce cell membrane permeability,and enhance the resistance of Arabidopsis thaliana to copper stress.The results could provide a theoretical basis for further analysis of the molecular regulatory network of plant response to copper stress.