华南农业大学学报2024，Vol.45Issue(6)：865-877,13.DOI:10.7671/j.issn.1001-411X.202406032
稻鱼共生系统土壤微生物群落组成和功能特征
Community structure and functional features of soil microbes in the rice-fish co-culture system
摘要
Abstract
[Objective]This study aimed to investigate the soil microbial community structure of the rice-fish co-culture system(RF),and compare its microbial functions on carbon and nitrogen cycling to those of rice monoculture system(RM).[Method]We sampled soil from paddies during the rice growth season at the site of the Qingtian rice-fish co-culture system,a globally important agricultural cultural heritage.Using high-throughput sequencing technology,we compared the structure and function of soil bacterial and archaeal communities between RF and RM.[Result]RF significantly increased the α-diversity of bacterial and archaeal communities,while the dominant phyla or genera remained the same as RM.RF also improved the interactions among soil microbes and the functional diversity of dominant network modules,but its effect on network stability was unclear.Based on FAPROTAX prediction of functions,RF strengthened the positive relationship between methane oxidation and nitrogen fixation compared to RM.However,RF weakened the relationship between nitrification and methane production/denitrification,as well as the relationship between methane oxidation and denitrification.The canonical correlation analysis results demonstrated that,compared with RM,RF reduced the effects of soil organic matter,available N,and available P contents on soil microbial community structure but enhanced the effects of soil total phosphorus content and soil pH.[Conclusion]In Qingtian,the traditional rice-fish co-culture system increases the α-diversity of soil microbial communities,global microbial interactions,and the complexity of microbial networks.However,traditional RF weakens the relationships between different microbial functional groups involved in methane and nitrogen cycling.These findings provide a basis for further research on the microbial ecological processes in rice-aquatic animal co-culture systems.关键词
稻鱼共生/土壤微生物/微生物网络/功能预测Key words
Rice-fish co-culture/Soil microbe/Microbial network/Function prediction分类
生物科学引用本文复制引用
刘瑛涵,任伟征,赵璐峰,郭梁,刘益曦,胡亮亮,陈欣..稻鱼共生系统土壤微生物群落组成和功能特征[J].华南农业大学学报,2024,45(6):865-877,13.基金项目
国家重点研发计划(2023YFD2401801) （2023YFD2401801）
浙江省"领雁"研发攻关计划(2022C02058) （2022C02058）
浙江省公益技术应用项目(LGN22C030002) （LGN22C030002）
浙江省"尖兵"研发攻关计划(2022C02008) （2022C02008）
