华南农业大学学报2024，Vol.45Issue(6)：898-907,10.DOI:10.7671/j.issn.1001-411X.202406044
海拔对稻田金背鲤肠道结构、消化酶活性和肠道菌群的影响
Effects of altitude on gut structure,digestive enzyme activity and gut microbiota of Cyprinus carpio var.Jinbei in rice field
摘要
Abstract
[Objective]In order to explore the gut structure,digestive enzyme activity and gut microbiota characteristics of Cyprinus carpio var.Jinbei at different altitudes.[Method]C.carpio var.Jinbei in rice fields at low(580 m),middle(830 m)and high(1340 m)altitudes were taken as the research objects.The histology,enzymology and molecular biology methods were used to compare and analyze the gut tissue structure,digestive enzyme activity and gut microbiota characteristics.[Result]The villus height of C.carpio var.Jinbei of the low altitude group(521.04 μm)was significantly higher than those of the middle altitude group(438.01 μm)and high altitude group(419.39 μm)(P<0.05).The trypsin activity of C.carpio var.Jinbei of the middle altitude group(4541.65 U/mg)was significantly higher than those of the low altitude group(4023.72 U/mg)and high altitude group(3722.10 U/mg)(P<0.05).Actinobacteria,Firmicutes,Proteobacteria,Chloroflexi and Tenericutes were the main gut microbiota of C.carpio var.Jinbei in rice field.[Conclusion]Different altitudes affect the intestinal morphology,digestive enzyme activity,and gut microbiota structure of C.carpio var.Jinbei in rice field.Although the gut microbiota of C.carpio var.Jinbei in rice field changes at different altitudes,it still maintains a relatively stable gut microbiota dominated by Actinobacteria,indicating that C.carpio var.Jinbei gut has been adapted to the ecological environment in rice field.关键词
海拔/稻田/金背鲤/肠道/消化酶/肠道菌群Key words
Altitude/Rice field/Cyprinus carpio var.Jinbei/Intestine/Digestive enzyme/Gut microbiota分类
农业科技引用本文复制引用
杨立,蒙庆米,白天泉,李嘉尧,姚俊杰,马兰..海拔对稻田金背鲤肠道结构、消化酶活性和肠道菌群的影响[J].华南农业大学学报,2024,45(6):898-907,10.基金项目
贵州省农业重大产业科学研究攻关项目(黔教合KY字[2019]013) （黔教合KY字[2019]013）
农业农村部稻渔综合种养生态重点实验室开放课题(2023-1) （2023-1）
